血管紧张素II诱导心肌成纤维细胞表达上调基因的分离和鉴定.pdfVIP

Acta Physiologica Sinica, October 25, 2005, 57 (5): 643-647 643 1 1,* 1 1 1 2 3 3 3 , 1 2 3 710061 710068 710032 (cardiac fibroblasts, CF)(angiotensin , Ang ) Ang CF CF (suppression subtractive hybridization, SSH) cDNA 1 9 7 (expressed sequence tags, EST)SSH CF Ang R363.2 + 1 Identification of up-regulated genes induced by angiotensin in cardiac fibroblasts 1 1,* 1 1 1 2 WANG Xin-Feng , GAO Guang-Dao , YANG Yu-Bai , ZHOU Juan , WANG Ya-Wen , SU Xing-Li , WANG Yan3 3 3 , HAN Feng-Chan , BAI Yu-Jie 1Department of Pathophysiology, Medical College of Xi’an Jiaotong University, Xi’an 710061, China; 2Department of Pathophysiology, Shaanxi Medical College, Xi’an 710068, China; 3Institute of Genetic Diagnosis, the Fourth Military Medical University, Xi’an 710032, China Abstract: To identify up-regulated genes in adult rat cardiac fibroblasts (CF) induced by angiotensin (Ang ), suppression subtractive hybridization (SSH) was performed between the CF stimulated by Ang (tester) and unstimulated CF (driver) to generate subtractive cDNA library. The library was screened with dot blots hybridization to further verify the differentially expressed cDNA clones. Partial positive clones (19 up-regulated genes) wer