HPA1-6, 15 基因多态性与急性心肌梗死相关性的研究.docVIP

HPA1-6,15基因多态性与急性心肌梗死相关性的研究 陈 赟 1,2，曾小菁1（550004 贵阳，贵阳医学院医学检验系血液教研室100195 北京，武警总部干休所2） [摘要] 目的 研究贵州地区人类血小板抗原（HPA1-6,15）基因多态性与急性心肌梗死（acute myocardial infarction，AMI61.05±10.27岁60.74±6.88岁OR=34.169，95%CI 80.221），而HPA1,3-6,15基因多态性差异无统计学意义（P0.05）；（2）影响AMI发病的多因素回归分析显示与对照组比较，AMI组中HPA1ab基因型差异无统计学意义（P0.05），但其在AMI发病的危险度比HPA1aa纯合子增加了4.256倍；HPA2aa基因型和HPA2b等位基因差异有显著统计学意义（P0.01，OR=0.148，95%CI 0.071～0.307和P0.01，OR=6.221，95%CI 3.201～12.091）。结论 （1）HPA1ab杂合子患AMI的危险度较HPA1aa纯合子增加；（2）HPA2aa纯合子可能是AMI发病的保护因子，而HPA2b等位基因可能是AMI发病的独立遗传性危险因素。 [关键词] 人类血小板抗原；基因多态性；急性心肌梗死；危险因素 [中图法分类号] [文献标志码] A Association of Human platelet alloantigens -1 to -6 and -15 polymorphisms with acute myocardial infarction Chen Yun, Zen Xiaojin(Clinical Laboratory Medicine,Guiyang Medical College, Gui Yang 550004, China) [Abstract] Objective To study the correlation between gene polymorphism of human platelet antigens (HPA1-6, 15) in Guizhou Province and the AMI. Methods 86 AMI patients (61.05 ± 10.27 years) , in line with 1979 WHO diagnostic criteria , parallel coronary angiography ; the control group, 85 cases (60.74 ± 6.88 years) , excluding cardiovascular diseases and thrombosis . To adopt the sequence-specific primer - polymerase chain reaction (SSP-PCR) amplification of the target gene , to carry on HPA genotyping , regression analysis of the risk factors in AMI . Results ( 1 ) compared with the control group , AMI group HPA2 gene polymorphism has statistically significant difference (P 0.01, OR = 26.36,95% CI 8.317 ~ 83.549), while HPA1 ,3-6, 15 gene polymorphism has no significant difference (P 0.05); (2) affect the incidence of AMI multiple regression analysis showed that compared with the control group , AMI group HPA1ab genotype has no significant difference (P 0.05), but its AMI disease risk increased by 4.3 -fold than HPA1aa homozygous ; HPA2aa HPA2b genotype and allele difference was statistically significant （P0.01，OR=0.148，95%CI 0.071～0.307和P0.01，OR=6.221，95%