HPA1-6, 15 基因多态性与急性心肌梗死相关性研究.docVIP

HPA1-6,15基因多态性与急性心肌梗死相关性研究 陈 赟 1,2，曾小菁1（550004 贵阳，贵阳医学院医学检验系血液教研室1；100195 北京，武警总部闵庄路干休所2） [摘要] 目的 研究贵州地区人类血小板抗原（HPA1-6,15）基因多态性与急性心肌梗死（acute myocardial infarction，AMI）的相关性。方法 选择AMI患者86例（61.05±10.27岁），符合1979年WHO诊断标准，并行冠脉造影证实；对照组85例（60.74±6.88岁），排除心脑血管疾病及血栓性疾病。采用序列特异性引物-多聚酶链反应(SSP-PCR)技术扩增目的基因，进行HPA基因分型，回归分析其在AMI中的危险因素。结果 ①与对照组比较，AMI组HPA2基因多态性差异有统计学意义(P0.01, OR=34.169，95%CI 14.553～80.221），而HPA1,3-6,15基因多态性差异无统计学意义（P0.05）；②影响AMI发病的多因素回归分析显示：与对照组比较，AMI组中HPA1ab基因型差异无统计学意义（P0.05），但其在AMI发病的危险度比HPA1aa纯合子增加了4.256倍；HPA2aa基因型和HPA2b等位基因差异有统计学意义（P0.01，OR=0.148，95%CI 0.071～0.307；P0.01，OR=6.221，95%CI 3.201～12.091）。结论 ①HPA1ab杂合子患AMI的危险度较HPA1aa纯合子增加；②HPA2aa纯合子可能是AMI发病的保护因子，而HPA2b等位基因可能是AMI发病的独立遗传性危险因素。 [关键词] 人类血小板抗原；基因多态性；急性心肌梗死；危险因素 [中图法分类号] [文献标志码] A Correlation of human platelet alloantigens -1 to -6 and -15 polymorphisms with acute myocardial infarction Chen Yun, Zen Xiaojing (Department of Hematology, Faculty of Laboratory Medicine, Guiyang Medical College, Guiyang, Guizhou, 550004, China) [Abstract] Objective To investigate the correlation between gene polymorphism of human platelet antigens (HPA1-6, 15) in Guizhou Province and acute myocardial infarction (AMI). Methods A total of 86 newly diagnosed AMI patients with a mean age of 61.05±10.27 years, who were in line with 1979 WHO diagnostic criteria, identified with coronary angiography and hospitalized in our affiliated hospital form April 2010 to October 2010 were enrolled in this study. Another 85 health individuals with a mean age of 60.74±6.88 and without any cardiovascular disease and thrombosis served as control. Sequence-specific primer-polymerase chain reaction (SSP-PCR) was employed to amplify the target gene, and genotype HPA gene. Regression analysis was used to analyze whether its polymorphism was the risk factors of AMI. Results Compared with the control group, HPA2 polymorphism in AMI group had significantly difference (P0.01, OR=26.36, 95% CI=8.317 to 83.549), while H