样品处理对肺移植术后患者全血中环孢素A浓度测定的影响.pdfVIP

维普资讯 样品处理对肺移植术后患者全血中环孢素A浓度测定的影响 何 晶(大连大学附属中山医院，大连市 116001) 中图分类号 R969．1；R978．11 文献标识码 A 文章编号 1001—0408(2008)02—0114—02 摘 要 目的：考察荧光偏振免疫 (FPIA)法测定肺移植术后患者全血中环孢素A(CsA)浓度时样品的保存温度、时间和不同稀释 方法对测定结果的影响。方法：将肺移植术后患者的全血标本分别置室温((22±2)℃)和冰箱(2～8℃)保存 1周或2周，考察其稳 定性；采用不同溶剂(灭菌注射用水、空白全血)稀释后与不经稀释的标本进行测定、对比，考察稀释条件。结果：室温或冷藏下的标 本测定结果均与保存前无统计学差异 (P 0．05)，但冷藏者变异小；2种方法稀释后测定结果与未稀释者无统计学差异 (P 0．05)，用空白全血稀释的结果最接近不稀释的值。结论：全血标本在室温下至少可稳定保存 2周，冷藏仍是最佳保存方法；空白全 血稀释标本最好。 关键词 环孢素A；荧光偏振免疫法；全血浓度；保存；稀释 InfluenceonDetermination ofCyclosporineW holeBloodSamplesW henPrepared HEJing(ZhongshanHospitalAffiliatedtoDalianUniversity，Dalian116001，China) ABSTRACT OBJECTIVE：Tostudythestorage(temperatureandduration)ofcyclosporinewholebloodsampleswhende— terminedbyFPIA andtheeffectofdifferentdilutionmethodonthedetermination results．METHODS：W holebloodsamples takenfrom patientswhohadundergonelungtranslationwereeitherstoredatroom temperature((22±2)℃)orcold—stored (2～8℃)for1or2weeksbeforestabilitytestwasperformed；samplesundergoingdilutionwithdifferentsolventssuchas sterilepyrogen——freewaterorblank plasmaandundilutedsamplesweredeterminedandcompared tOinvestigatethedilution condition．RESULTS：Therewasnosignificantdifferenceforthedeterminationresultsforsamplesstoredatroom temperature orcold—storedascomparedwithbeforestorage(P0．05)，however，thecold—storedsamplehadlessvariation；andtherewas nosignificantdifferenceforthedeterminationresultsofthesamplesafterbeingdilutedbytwodifferentdilationmethodsas comparedwithundilatedsample(P 0．05)，withthedeterminationresultofthewholeblood—dilutedsamplemoresimilartO thatoftheundiluatedone．C0NCLUS10N ：W holebloo dsampleofcyclosporinecanbestoredstablyatroom temperatureat leastfor2weeks．ColdstorageiSthebeststoragemethodandblankwholebloo dwasprovedtObethebestdilutionsolvent． KEY WORDS Cyclosporine；FPIA；W holebloo ddrugconcentration；Storage；Dilution 环孢素 A(CsA)具有选择性免疫抑制功能，广泛用于器官 察 ，以便为临床医师及患者提供更好的、更可靠的药学服务。 移植后排斥反应和 自身免疫性疾病。荧光偏振免疫 (FP