一种有效的蚜虫基因组DNA提取方法73625.pdfVIP

林 业 科 学 研 究　2005, 18 ( 5) : 64 1～封三 F ores t R esea rch 　　文章编号 : 100 11498 (2005) 05064 103 一种有效的蚜虫基因组 DNA 提取方法 杨子祥 , 冯 　颖 , 陈晓鸣 (中国林业科学研究院资源昆虫研究所 ,云南 昆明　650224 ) 关键词 :蚜虫 ;基因组 DNA ;提取方法 中图分类号 : Q523　　　文献标识码 : A An Effective M ethod for Extraction Genom ic D NA from A ph ids YAN G Z ix iang, FEN G Ying, CHEN X iaom ing ( The R esearch In stitu te of R esou rce In sects, CA F, Kunm ing　650224 , Yunnan , Ch ina) Ab stract:Aph id is a group of sm all in sects in the Homop tera. There are app roxim ately 4 000 aph id sp ecies all over the world. M any of them attack important agricu ltu ral crop s and therefore have econom ic importance. However, som e sp ecies such as Ch inese gallnu t aph id s are beneficial for hum an beings. Mo st ap h id s disp lay comp lex life cycle s w ith alternation of sexual and a sexual generation s and ho st p lant alternation. Molecu lar genetic m arkers have good stab ilitie s, h igh variab ility and mo stly are free from environm ental influence s. So they were very su itab le for aph id research and cou ld tack le som e p rob lem s wh ich the traditional taxonom ical m ethod s cou ld not solve. The p ap er introduced a simp le and effective m ethod that cou ld extract genom ic DNA from a single aph id individual and cou ld be u sed for the re search of molecu lar genetic m arker such as RA PD , RFLP and DNA sequence determ ination. Key words: ap h id, genom ic DNA , extraction m ethod 蚜 虫 属 于 同 翅 目 ( Homop tera ) 蚜 总 科 来 ,应用分子遗传标记技术 ,从 DNA 水平上研究蚜 ( ) ( ) Ap h ido idea 和球蚜总科 A delgo idea