急性脑出血中医辨证分型与血清可溶性细胞间黏附分子和丙二醛含量的相关性研究.pdfVIP

维普资讯 · 212 · JTCM．Mar．2005，Vo1．46，No．3 证 的 研 究 急性脑出血中医辨证分型与血清可溶性细胞间 黏附分子和丙二醛含量的相关性研究 陈少芳 梁 晖。 摘要 目的：探讨不 同中医辨证分型急性脑 出血患者血清可溶性细胞间黏附分子(sICAM一1)和丙二醛 (MDA)含量的变化及 意义。方法：采用双抗体夹心酶联免疫吸附(ELIsA)法、硫代巴比妥酸(TBA)法分别测定8O例急性脑出血患者和 2O例健康人 血清中sICAM一1、MDA的含量。结果：急性脑出血患者血清slCAM一1、MDA含量明显升高。中脏腑组两指标较中经络组显著 增高，中经络实证组较中经络虚证组显著增高，中经络各组以痰热腑实组升高最为显著，72小时内以25～48小时组为最高。 结论：急性脑出血 白细胞黏附、机体氧化应激增强，患者血清 sICAM一1、MDA 明显升高，二者关系密切 ，参与了脑出血后脑损 伤的病理过程；两指标水平的差异可能是中风出现不同证型的内在因素之一；急性脑出血两指标水平的高峰期在 25～48小 时；两指标可作为判定病情严重程度的参考指标。 主题词 脑出血 ／8液 丙二醛 ／血液 辨证分型 StudyonCorrelativityofTCM SyndromeTypesofAcuteCerebralHemorrhagewithsICAM 一1andM DA Contents ChenShaofang ，LiangHui (1．TCM Department，FujianCollegeofTCM ，Fuzhou350003；2．People’HospitalA，jiatedtoFujianCollegeofTCM) Objective：Toexplorechangesandsignificanceofserum solubleintercellularadhesionmoleculae(sICAM一1)andmal0ndiadehyde (MDA)contentsinpatientsofacutecerebralhemorrhageofdifferentTCM syndromes．Methods：Serum siCAM一1and MDA contentsweredeterminedrespectivelybydoublesandwichenzyme—linkedimmunosorbantassay(ELISA)andthiobarbituricacid (TBA)methodin80casesofacutecerebralhemorrhageand 20healthypersons．Results：Serum sICAM一1andMDA contents increasedsignificantlyinthepatientofacutecerebralhemorrhage，andincreasesofthetwoindexesinthegroupofapoplexy involving theZang—。and Fu—。organswere significantly higher than those in thegroup ofapoplexy involving channelsand collaterals，andin thegroupofexcesssyndromeofapoplexyinvolvingchannelsandcollateralgroupweresignificantlyhigherthan thoseinthegroupofdeficiencysyndromeofapoplexyinvolvingchannelsandcollaterals．Amongthevariousgroupofinvolving channelsandcollaterals，thephlegm—heatandfu—organexcessgroup increasedmostob