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Alb4 of Arabidopsis Promotes Assembly and Stabilization of a Non Chlorophyll-Binding Photosynthetic Complex, the CF1CF0-ATP Synthase.pdf

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Alb4 of Arabidopsis Promotes Assembly and Stabilization of a Non Chlorophyll-Binding Photosynthetic Complex, the CF1CF0-ATP Synthase.pdf

MolecularPlant ·Volume2 ·Number6 ·Pages1410-1424 ·November2009 RESEARCHARTICLE AIb4ofArabidopsisPromotesAssemblyand StabilizationofaNonChlorophyll--Binding PhOtOsyntheticComplex，theCF1CFo-ATP Synthase MoniqueBenz一，ThomasBals，‘IreneLuiseGiigel一，MarkusPiotrowski，‘AndreasKuhnd， DanjaSch~inemann，‘J0rgenSoil。，bandElisabethAnkele。，’ aDepartmentofBiologieI-Botanik．8iozentrum Ludwig—Maximillians—UniversityMunich．Grosshadernerstrasse2_4，D一82152Planegg—Martinsried，Germany b MunichCentreforIntegratedProteinScienceCIPSM，Ludwig—M aximillians—UniversityM unich，Feodor—Myhen—Strasse25，D一81377Munich，Germany cDepartmentofPlantPhysiology,Ruhr-UniversityBochum，D-44780Bochum．Germany d DepartmentofM icrobiolog~ UniversityofHohenheim．Garbenstrasse3O，D·70599Hohenheim，Germany ABSTRACT AllmembersoftheYidC／Oxal／AIb3proteinfamilyareevoIutiOnariIyconservedandappeartofunctionin membraneproteinintegrationandproteincomplexstabilization．Here．wereportonasecondthylakoidalisoform of AIb3．namedAIb4．AnalysisofArabidopsisknockoutmutantIinesshowsthatAIb4isrequiredinassemblyand／orstability oftheCF1CF0．-ATPsynthase(ATPase)．alb4mutantIinesnotonlyhavereducedsteady-stateIevelsofATPasesubunits，but alsotheirassemblyintohigh—molecular-masscomplexesisaltered．1eadingtoareductionofATPsynthesisinthemutants． Moreover,weshowthatAIb4butnotAIb3physicallyinteractswiththesubunitsCF1pandCF0U．Summarizing，thedata indicatethatAIb4functionstostabilizeorpromoteassemblyofCF，duringitsattachmenttothemembrane．embedded CF0part． Keywords： AIb4；AIb3；Arabidopsis；thylakoidmembraneprotein insertion；proteincomplexassembly；CFICFo-ATP synthase． INTRODUCTION eta1．，2OO5)．RNAi—mediatedknockdownofOxa1I，thehuman Recently,an evoIutionarilyconservedproteinfamiIvOfinte． homolog。resultsindecreased evelsoftheF1F0．_ATPsynthase gralmembraneproteins，theAIb310xa1／YidC proteins，was and ofNADH：ubiauin0ne oxdoreductase (Stiburek