Expressions of genes related to genome stability and DNA repair in nasopharyngeal carcinoma clustering families.pdfVIP

Expressionsofgenesrelatedtogenomestabilityand DNA repairinnasopharyngealcarcinomaclustering fam ilies* XiaojunZhou，DaofaTian，ShizhenWang，YanRuan。，BaoshanO3u。，LijuanZhang，BiaoqingLu TheE．N．TDepartmentofZhongshanHospitalA陆liatedtoGuangzhouUniversityofTraditionalChineseMedicine． Zhon~shan528400，China TheCombineTraditionalChineseandW esternMedicineDepartmentofHunanUniversityofTraditionalChinese Medicine，Changsha410007，China 0TheE．N．TDepartmentofFirstHospitalA陆liatedtoGuangzhouUniversityofTraditionalChineseMedicine． Guangzhou510405，China Received：10October2009／Revised：26October2009／Accepted：5November2009 Abstract Objective：TheaimoflhestudywasloobservetheexpressionsofgenesrelatedtogenomestabilityandDNA repairInlhemembersofnasopharyngealcarcinoma(NPC)clusteringfamilies．Methods：lntheZhongshanCiytwherelhere ishighlyincidencerateofNPC，wechosethemembersoflheNPCclusteringfamiliesasobjects．andthepatientsofnaso． pharyngitisandNPCasthecontrolgroup．WeisolatedtheRNA仃0mlhenasopharyngeallissue．andsynthesizeditscRNA． thegenomestabilityandDNArepairgeneschiptechnique．chemIluminescenldetectionandrea1．timefluorescencequantita． 1ivetechniquewereusedtoexaminethegenomestabilityandDNArepairgenesinthenasopharyngeaItissue．Results：More genomestabiliytandDNArepairgeneswereup—regulatedjnlhemembersoflheNPCclusteringfamilieslhantheNPCpa． 1ients，andtherangeofup-regulatedwashigh，withlheoverup-regulated100timesgenesincludingTEP1，MSH4．PM$2L1． FewergenomestabiliytandDNArepairgenesweredown-regulatedinthemembersoftheNPCclusteringfamiliesthanthe NPCpatients，theubiquitingenesalmoslweredown-regulated，theresultsalsocouldbeconfirmedbyreal-timefluorescence quantitativePCR．Conclusion：Therearespeciallyexpressioncharacterofgenomestabi,ytandDNArepairgenesinthe membersofNPCclusteringfamilies． Keywords nasopharyngealcarcinoma(NPC)；cancercl