利用SSR标记和表型性状聚类分析食味优良粳稻多样性.pdfVIP

中国水稻科学 (ChinJRiceSci)，2009，23(6)：573～582 httpt|| w．ricesci．cn DOI：10．3969／j．issn．1001-7216．2009．06．03 573 利用SSR标记和表型性状聚类分析食昧优 良粳稻多样性 张春红1 李金州1，2 朱 镇1 张亚东1 赵 凌1 王才林1， (江苏省农业科学院粮食作物研究所 ／江苏省优质水稻工程技术研究中心 ／国家水稻改良中心南京分中心，江苏 南京 210014；z南京农业大 学 农学院，江苏 南京 210095； 通讯联系人 ，E—mail：clwang@jaas．ac．cn) ClusterAnalysisonGoodEatingQualityjaponicaRice(Oryzasativa)BasedonSSRMarkers andPhenotypicTraits ZHANGChun—hong ，LIJin—zhou，。，ZHuZhen ，ZHANGYa—dong ，ZHAOLing ，WANGCai—lin ， (InstituteofFoodCrops，JiangsuHighQualityRiceR D Center，NanjingBranchofChineseNationalCenterforRiceImprovement。 JiangsuAcademyofAgriculturalSciences，Na ing210014，China；。CollegeofAgriculture，Na ingAgriculturalUniversity，Nanjing 210095，China； Correspondingauthor，E-mail：clwang@jaas．ac．cn) Abstract：Diversityanalysiswasperformedonconventionaljaponicariceaccessionswithgoodtasteathomeandabroad basedonSSRmolecularmarkers，agronomictraitsandtastecharacteristics．A tota1of290allelesin60accessionsweredetec— tedat72SSR lociwiththehighsimilaritycoefficientsvaryingfrom 0．600to0．924．Chromosome5showedthegreatestvalue inaverageallelenumber．Additionally，mostoftheSSR 1ocicoulddetect3to4alleles．AnUPGMA dendrogram basedonthe clusteranalysisofthegeneticsimilaritycoefficientsshowedthatthegroupingtrendofpartialaccessionswasgeographic—relat— edandmostoftheaccessionsfromJiangsuProvince，Chinawereclusteredtogether．Furthermore，manydomesticaccessions from thesouthandnorthofChinaweregeneticallysimilartotheforeignjaponicavarieties，aswasprovedbytheirpedigree from theforeignhigh-qualitysources．Accordingtotastecharacteristics，partialaccessionswithexcellenttastewereclearly clusteredintoonecategorythoughtheycamefrom differentgeographicalregions，whichindicatesthattastecharacteristicsof somevarietiesweremainlydeterminedbytheirgeneticbasis．Inaddition，theagronomictraitsofjaponicawithgoodtastemay havesomecloserelationshipwith t