H5N1亚型禽流感灭活疫苗种毒Re-6株的生物学特性及免疫原性研究.pdfVIP

H5N1亚型禽流感灭活疫苗种毒Re—6株的生物学 特性及免疫原性研究 曾显营1田国彬1冯华朋1邓国华1施建忠1张盼涛1苏文1杨婧1 刘景利2徐佳2陈化兰1 1.中国农业科学院哈尔滨兽医研究所，兽医生物技术国家重点实验室， 农业部动物流感重点开放实验室，国家禽流感参考实验室，哈尔滨 150001 2.哈尔滨维科生物技术开发公司，哈尔滨 150001 摘要：本研究对经反向遗传方法构建的重组禽流感H5N1亚型变灭活疫苗种毒Re—6株的生 物学特性及免疫效力进行研究。将Re—6株接种SPF鸡胚后37℃培养72h，鸡胚存活，且无病变， HA滴度达210；以0.1ml(106.0EID50/0.1ml)的剂量鼻腔感染4周龄SPF鸡10天后血清HI抗体转 阳，无任何症状，也不排毒；SPF鸡静脉致病指数(IVPI)为0；以Re—6重组株为种毒制备灭活疫苗， 免疫SPF鸡和SPF鸭后，3周时平均HI抗体效价达8.310g2和8.4log2；免疫鸡和免疫鸭对亲本强 毒株GD1322攻击提供完全保护。以上结果表明禽流感灭活疫苗种毒Re—6株对SPF鸡胚和SPF 鸡无致病性、适合鸡胚增殖、抗原针对性强，且该毒株制备的灭活疫苗具有良好的免疫效力，是理想的 疫苗种毒株。 关键词：禽流感；H5N1;Re—6株；灭活疫苗 ThebiologicalcharacteristicsandimmunogenicityofaH5N1inactivated vaccineseedvirusstrainRe6generatedbyreversegenetics ZENGXianying1 FIANGuobin1FENGHuapeng1DENG GuohuaSHIJianzhong1 ZHANGPantao1SUWen1YANGJing1LIUJingli2XUJia2CHENHualan1 1.StateKeyLabofVeterinaryBiotechnology/AnimalInfluenzaLaboratoryoftheMinistryof Agriculture,HarbinVeterinaryResearchInstitute,CAAS,Harbin150001; 2.HarbinWeikeBiotechnologvDevelopmentCompany,Harbin150001 Abstract:Aninactivatedvaccineseedstrain(Re-6)hasbeengeneratedbyreversegeneticsfrom theavianinfluenzavirus(GS1322).Inthisstudy,weevaluatedthebiologicalcharacteristicsand immunogenicityofRe-6strain.WhenSPFembroynatedchickeneggswereinoculatedwithRe-6 strainandincubatedat37Cfor72hours,alltheeggswerealiveandtheHAtiterineggscouldreach to210.Novirussheddingcouldbedetectedformeithertracheaorcloacaswabsafter4-weekSPF chickenswereinoculatedintranasallywith106.0EID50ofRe-6strain,andallthechickenhad seroconvertedbyday10p.i..IVPIvalueofRe-6was0.AninactivatedvaccinederivedfromRe-6 straincouldinducehighHIantibody(8.31og2and8.41og2,respectively)inSPFchickensandducks3 weekspost-immunization(P.V.);theimmunizedchickensandduckscouldbecompletelyprotected fromthehomologousvirusGD1322.Thenonpathogenic,highgrowthandgoodimmune