细胞传代培养与MTT法检测化学药品对体外培养细胞增殖及存活的影响.docxVIP

Cellularsubculture and the effect of MTT assay of scanning of peroxideto the growth and survival of cells in vivo.HuBoqiang1Dong ZhiWei111School of Life Science, Beijing Institute of Technology, Beijing 100081,ChinaCellular subculture assays is the rudimental method of all cellular experiments. Via subculture, cells could survive, duplicate and preserve their own genes. In our experiment, we transferred the subculture cells to 96-well plate, treating with H2O2 in different concentrations and dying living cells (6251 cell line) with MTA. Finally, we got the effect of H2O2 in