茶花多酚类物质的LC-MS鉴定及抗氧化活性研究论文.pdfVIP

茶花多酚类物质的LC-MS 鉴定及抗氧化活性研究 1 2 1 1 屠幼英 ，程 龙 ， 杨子银 ， 徐 懿 （1.浙江大学茶学系，杭州 310029；2.江苏省联合职业技术学院无锡卫生分院, 江苏无锡 210024 ） 摘要：通过液质联用分析从茶花乙酸乙酯层中鉴定出4 类化合物：a)儿茶素类：儿茶素、表儿茶素、没食子儿茶 素、表没食子儿茶素、儿茶素没食子酸酯、表儿茶素没食子酸酯、没食子儿茶素没食子酸酯、表没食子儿茶素没 食子酸酯；b)儿茶素衍生物：儿茶素糖苷物与儿茶素二聚体；c)黄酮苷类：山奈酚、毛地黄黄酮与槲皮素单糖苷 及双糖苷类物质。经HPLC 分离与DPPH 测定，确定表没食子儿茶素没食子酸酯与表儿茶素没食子酸酯为茶花中 主要抗氧化活性成分，在茶花抗氧化活性中起到主要的作用。 关键词：茶学；茶花；抗氧化活性；液质联用；儿茶素；黄酮苷 Liquid Chromatographic-Mass Spectrometric Analysis of Phenolics and Evaluation of the Antioxidant Compounds of Extracts from Tea Flower (Camellia Sinensis) TU Youying 1, CHENG Long 2, YANG Ziyin 1, Xu Yi 1 （1 Department of Tea Science, Zhejiang University, Hangzhou 310029,China; 2 Wuxi Higher Health Vocational Technology School , Wuxi 210024, Jiangsu ,China ） Abstract: High performance liquid chromatography (HPLC) combined with diode array (DAD) and electrospray (ESI)-ion trap-MS detection was used to separate and identify the compounds present in EEA. Eight catechins, namely, catechin, epicatechin (EC), gallocatechin, epigallocatechin, gallocatechin gallate, epigallocatechin gallate (EGCG), catechin gallate and epicatechin gallate (ECG), catechins derivatives, namely, EC-3 -di- rhamnoside and catechins dimer, and flavonol glycosides, namely, quercetin, luteolin, kaempferide and kaempferol mono-, diglycosides (glucoside and rhamnoside) were identified. In addition, it is found that EGCG and ECG are the major active components responsible for the antioxidant activity of tea flowers by HPLC separation and DPPH assay. Keywords: tea science, tea flower, antioxidant activity, LC-MS