昆虫热休克蛋白与研究进展.pdfVIP

中国农业科学 2005,38(10):2023-2034 Scientia Agricultura Sinica 昆虫热休克蛋白的研究进展 王海鸿，雷仲仁 （中国农业科学院植物保护研究所植物病虫害生物学国家重点实验室，北京 100094 ） 摘要：热休克蛋白是细胞或生物体受到热胁迫后新合成的一类遗传上高度保守的蛋白，在原核生物和真核生 物中普遍存在。作者根据国内外近年来热休克蛋白的研究概况，对昆虫的主要热休克蛋白家族的生物学特性、辅 助蛋白、热休克基因的调控和热激信号的传导进行了综述，分析了热休克蛋白在昆虫体内作为分子伴侣、诱导生 物体耐热性和稳定细胞骨架的生物学功能，并讨论了昆虫热休克蛋白在衰老和生物进化研究中的意义以及在生物 标记和肿瘤治疗上的应用前景。 关键词：胁迫蛋白；Hsp；热休克基因；果蝇；昆虫 Current Developments of Heat Shock Proteins in Insect WANG Hai-hong, LEI Zhong-ren (State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Acadey of Agricultural Sciences, Beijing 100094 ) Abstract: Heat shock proteins, or Hsps, are a small number of highly conserved protein synthesized in cultured cells or whole organisms, which exposed to elevated temperatures. It has been observed in every organism in which it has been sought, from prokaryote to eukaryote . This paper briefly reviews recently studies and focus on the traits of the major hsps family, cohorts assisting hsps , regulation of heat shock gene expression, and the possibility of signal transduction pathways. And then analyses the functions of hsps with regard to being molecular chaperone, inducing thermotolerance and stabilizing cytoskeleton. Aging and evolutional importance, biomarker and therapeutic application of Hsps are also discussed. Key words: Stress protein; Hsp; Heat shock gene; Drosophila; Insect 热休克蛋白（heat shock proteins ，Hsps ）是细胞 高，与细胞的分化、发育密切相关；Hsps 在正常生理 或生物体在一定时间(几小时、几分钟、甚至几秒钟) 条件下不存在或表达量低，但能为热强烈诱导，具有 内遭受高于其正常生长温度 8～12℃（一般称为亚致 保护细胞的功能。Hscs 和 Hsps 在核苷酸序列上高度 死温度 sub-lethal temperature ）以上的温度时，新合成