葛根素对低O高CO肺动脉高压大鼠肺动脉平滑肌细胞线粒体途径凋亡的影响.docVIP

葛根素对低O2高CO2肺动脉高压大鼠肺动脉平滑肌细胞线粒体途径凋亡的影响 作者：丁城 作者单位：温州医学院第一附属医院 呼吸内科，浙江 温州 325000 【摘要】 目的: 研究在慢性低O2高CO2情况下，葛根素对肺动脉平滑肌细胞(PASMCs)线粒体途径凋亡的影响。方法:雄性SD大鼠40只，随机分为正常对照组(N组)、慢性低O2高CO2 2周组(H2w组)、慢性低O2高CO2 4周组(H4w组)、慢性低O2高CO2 4周+葛根素组(H4w+G组)，每组10只。TUNEL法检测PASMCs凋亡;免疫组化测定PASMCs线粒体细胞色素C(Cyt-c)释放量及胞浆Bcl-2、Bax含量;分光光度法检测肺组织匀浆caspase 9酶活性。结果:H2w组、H4w组和H4w+G组mPAP、RV/(LV+S)、PAMT、PASMCs胞浆 Bcl-2含量均显著高于N组(P 0.01)，Cyt-c释放量、Bax含量及肺组织匀浆caspase 9酶活性均显著低于N组(P 0.01);H2w组、H4w组各级PASMCs和H4w+G组肺弹力型动脉和肺微细动脉PASMCs凋亡指数显著低于N组(P 0.01)。H4w+G组mPAP、RV/(LV+S)、PAMT、肺弹力型动脉和肺微细动脉PASMCs 中Bcl-2含量均显著低于H4w组(P 0.01);弹力型动脉和肌型动脉PASMCs凋亡指数、Cyt-c释放量，肺组织匀浆caspase 9酶活性均显著高于H4w组(P 0.01);Bax含量在H4w+G组与H4w组间差异无显著性(P 0.05)。结论:葛根素有抑制慢性低O2高CO2肺动脉高压和肺血管重建的作用，促进PASMCs通过线粒体途径凋亡可能是其作用机制之一。 【关键词】 低氧 慢性 高血压 肺性 线粒体 凋 Effects of Puerarin on mitochondrial-dependent apoptosis pathway in pulomnary hypertension induced by hypoxic hypercapnia DING Cheng,WANG Liang-xing,CHEN Shao-xian,HUANG Xiao-ying. Department of Respiratory Medicine, the First Affiliated Hospital of Wenzhou Medical College, Wenzhou, 325000 Abstract: Objective: To study the effects of puerarin on mitochondrial-dependent apoptosis pathway in pulomnary hypertension induced by hypoxic hypercapnia. Methods: Fourty Sprague-Dawley rats were randomly divided into four groups: normal control group(N)，hypoxic hypercapnic 2 weeks group (H2w)，hypoxic hypercapnic 4 weeks group (H4w) and hypoxic hypercapnic 4 weeks + Puerarin group (H4w+G). The apoptsis of PASMCs was tested with TUNEL. The release of Cyt-c from mitochondria and the content of Bcl-2 and Bax in cytoplasm were determined with immunohistochemistry. The activity of caspase 9 was detected with spectropho-tometric method. Results: ①The mPAP, RV/(LV+S), PAMT and the content of Bcl-2 of PASMCs of group H2w， group H4w and group H4w+G were significantly higher than those of group N (P 0.01). The release of Cyt-c，the content of Bax of PASMCs and the activity of caspase 9 of lung in group H2w, group H4w and group H4w+G were si