猪腰豆幼苗中乙酰胆碱酯酶和酯酶活性及其对农药的敏感性.pdfVIP

※基础研究 艮品科学 2011，V01．32，No．1139 猪腰豆幼苗中乙酰胆碱酯酶和酯酶活性 及其对农药的敏感性 纪淑娟，秦奉达，白冰 1 (沈阳农业大学食品学院，辽宁沈阳 10161) 摘要：选取猪腰豆为研究对象，测定不同时期、不同部位猪腰豆幼苗中乙酰胆碱酯酶(ACHE)和酯酶活性，选取 活性最高的部位提取酶，分别检测其对有机磷及氨基甲酸酯类农药的灵敏性，确定最低检测限。结果表明：AChE 酯酶，对敌敌畏、氧化乐果、灭多威的最低检测限分别为0．025、0．07、0．07mg／kg，低于国家规定的允许残留 限量，可以满足农药残留快速检测的要求。 关键词：乙酰胆碱酯酶；酯酶；猪腰豆；有机磷类农药；氨基甲酸酯类农药 andEsteraseActivitiesin Bean andTheir toPesticides Acetylcholinesterase KidneySeedlings Sensitivity JIShu-juan，QINFeng—da，BAIBing ofFood 110161，China) (CollegeScience，ShenyangAgriculturalUniversity，Shenyang Abstract：In吐li8activitiesof e强teIaScin bean andtheir paper,the acetylcholinesterase(ACHE)andkidneyseedlingssensitivity that to were indicatedtheactivitiesofAChEandesterasewe犯varieddifferent pesticidesanalyzed．Results during germination and of bean ofAChEwasthe after6 of growthperiodskidneyseedlings．Thespecificactivity highest daysgermination，which 1．9433 wasupt00．969U／mL，andtheestcrascactivityreachedupto old bean Wasmoresensitiveto thantheesterasefrom bean the kidneyseedlings pesticides E-day-oldkidneyseedlings，and detectionlimitsfor dimethoateand WCTC and0．07 dichlorvos，oxidized 0．025，0．07mg／kg mg／kg，respectively，which methomyl themaximum residue bean Canmeetthe wereallbelow allowable derivedfrom limits．Therefore，AChEkidneyseedlings for dete