大鼠膀胱出口梗阻致膀胱重构的形态结构研究_刘冲.pdfVIP

基础研究 大鼠膀胱出口梗阻致膀胱重构的形态结构研究 刘冲,王东文,高宏飞,荆强,史骁 （山西医科大学第一医院泌尿外科，山西太原 030001） Morphological structure in the bladder reconstruction of bladder outlet obstruction of rats * LIU Chong, WANG Dong-wen , GAO Hong-fei, JING Qiang, SHI Xiao (Department of Urology, the First Hospital of Shanxi Medical University, Taiyuan 030001, China) ABSTRACT: Objective To observe the proliferation and apoptosis of the bladder detrusor cells in different periods of rat bladder outlet obstruction and the change of interstitial collagen fiber. Methods Bladder outlet obstruction models were established in 40 Wistar rats, which were divided into 3 groups, including the sham-operation group (n=10), 2-week obstruction group (n=15) and 4-week obstruction group (n=15). The PCNA expression was analyzed with immunohistochemical method, the apoptotic index was detected with TUNEL, and the changes of collagen fiber in the bladder detrusor were observed with VG staining. Results The expression of PCNA was (17.19±1.37)%, (50.74±3.15)% and (33.58±2.80)% in the sham-operation group, 2-week obstruction group and 4-week obstruction group, respectively, with significant difference among the groups (P 0.05). The apoptotic index was (20.22±1.70)%, (33.90±1.86)% and (51.18±3.30)% respectively, with significant difference among the groups (P 0.05). The proportion of collagen fiber in the bladder detrusor was (7.07±0.67)%, (13.19±1.50)%, and (22.98±2.89)%, respectively, with significant difference among the groups (P 0.01). Conclusions Changes of shape and structure of bladder detrusor in different periods of bladder outlet obstruction may be caused by cell proliferation, apoptosis, and changing proportion of interstitial collagen fiber. KEY WORDS: bladder outlet obstruction; detrusor; PCNA; TUNEL; apoptosis index; collagen fiber 摘要:目