苦参碱聚乳酸微球的缓释性和玻璃体腔注射的安全性研究.pdfVIP

ChinOphthalRes，January2010，Vo1．28，No．1 · 实 验 研 究 · 苦参碱聚乳酸微球的缓释性和玻璃体腔注射的 安全性研究 刘丹岩 马景学 曹德英 王建欣 刘建宗 吕兰存 Sustained releaseability and safety ofmatrine polyactic acid microsphere intravitrealinjection LiuDanyan，MaJingxue，CaoDeying，WangJianxin，LiuJianzong，LaLancun．DepartmentofOehthalmology， AffiliatedSecondHospitalofHebeiMedicalUniversity，Shijiazhuang050000，China Abstract Background Theanti—proliferativeeffectofmatrinehasbeendemonstratedanditsrelevancetopreventionand treatmentofproliferativeretin0vitre。pathyisconcerned．However，theintravitreousinjectionoffree-matrinereiterativelymayraise theriskofocularinfection． Objective Thegoalofthepresentstudyistoinvestigatethesustainedreleasingabilityandsafety ofmatrinepolyacticacidmicrosphere(MAT—PLA—MS)intravitrealinjection． Methods MAT—PLA—MSwaspreparedbyHebei MedicalUniversity and examined underthe transmission electron microscope．The release ofMAT—PLA—MS wasmonitored by ultravioletspectroph0t0mety．Free—matrinewiththedoseof1，2，4mgwasintravitreallyinjectedrespectivelyin12eyesofNew Zealandalbinorabbitsinfree—matrinegroup，andMAT—PLA—MSwithmatrine(2，4，6mgrespectively)wasadministeredin16eyes separatelyinmatrinemicrospheregroup．Theblankmicrospherewasinjectedin6righteyesasblankcontrolgroupandnormal salinesolutionwasinjectedin6fellow eyesascontrolgroup．Theretinalfunctionchangewasevaluatedbyelectroretin。gram (ERG)，andthemorphologicalandhistologicalchangeofretinafollowingdruginjectionwereassessedundertheslitlamp biomicroscope，indirectophthalmosc0pe，lightmicroscopeandtransmissionelectronmicroscope．ThedecomposedprocessofMAT— PLA-MS invitreouswasrecorded with ocularanteriorsegmentandfunduscolorcamera． Results MAT—PLA—MS containing matrineshowed thespher