碳源对Bacillus cereus CZ氨肽酶产量及糖代谢酶活性的影响.pdfVIP

现代食品科技 ModernFoodScienceandTechnology 2014，Vo1．30，No．6 EffectsofCarbonSourcesonProductionofLeucine AminopeptidaseandActivitiesofSugarM etabolicEnzymes ProducedbyBacilluscereusCZ cuiTang-bing，JI『ANGLI-xiang,HANQian，LIJun—xia (SchoolofBioscienceandBioengineering，SouthChinaUniversityofTechnology,Gunagzhou510006，Chnia) Abstract：Leucineaminopeptidase(LAP)producedbyBacilluscereusCZwasinvestigatedinthisstudy．Resultsnidicatedthat L-arabniosewasthebestcarbonsourceforproducnigLA followedbyD-galactoseandlactose，whereasglucose，maltose，sucrosenadsodium bicrabonateobviouslyinhibitedLAPproduction．TheproductionofLAPwasalsoreducedlargelywhenL-arabniosewasmixedwiht ohter simplesugars，orLactose／D·galactosewasmixedwiht glucose．Th emixtureoflactosenadglucosewashteoptimalcrabonsourcesforcell growth．Whenusing2％ lactose，D—galactosenadL—raabnioseascrabonsources，LAPactivitiesdidnotreachhtemaximmu valuesafter48h fermentation．Forhtesugrameatbolicenzymes，31-galactosidasecnabestronglyinducedbylactose．L-Arabnio~hadcertainniductioneffect, whileD-galacoteshadastrongdepressioneffect~Galactokinasewas inducedsignificnatlybyD-galacotse,followedbyL-arabnioesandlacotse． L wasniducedsrtonglybyL-arabinose．moderatelybyohtersimplesugarsnadmixedsugars．LAPproductionreachedhte highestwiht4％ L·arabinosenadD-galactose．Extracelluargalactokinasedecreasedwiht nicreasinghtesugra concentration．butintracellular enzymesshowedhtereversetrend．L-arabnioseisomeraseincreasedwiht increasingconcenrtationofL-arabniose．LAP productionreachedhte highestwhenfemr neattionfor56hwiht 4％L-arabniose．However,itsproductionincreasedcontinuouslywihtfermenattiontimewhen4％ D-galactosewasused． Keywords：leucnieaminopeptidase；13-galactosidase；galactokinase； ；Bacilluscereus ArticleNo．：1673-9078(2014)6-158—167 碳源对Baci／／usCeFeLiSCZ氨肽酶产量及