不同颜色染发剂对蚕豆根尖细胞微核的影响.docVIP

不同染发剂对蚕豆根尖细胞微核的影响 (10生本 10112314153 郑苏瑾) 前言：随着人们生活水平和美容要求的不断提,高, 染发剂的使用越来越普遍。随着人们生活水平和美容要求的不断提高, 染发剂的使用越来越普遍。这些染色剂是否具有致突、致畸、致癌作用, 已成为人们日益关注的问题。蚕豆根尖细胞微核技术是一种以染色体损伤及纺锤丝毒性等为测试终点的植物体细胞检测方法,因具有材料方便,培养简单、技术容易掌握、准确、快速、有明显剂量-效应等优点而成为目前研究和应用较多的检测环境污染物质的检测系统[13]。为了给染发剂的研究制造和管理使用提供科学依据, 我们选用目前畅销的一些染发剂, 用蚕豆根尖细胞微核试验。测定蚕豆根尖细胞中出现的微核率,以此分析染发剂的诱变性,从而判断其对遗传物质是否有毒性效应。我们用不同的染发剂处理蚕豆根尖细胞，统计微核千分率（微核细胞率）。 关键词：染发剂;蚕豆根尖;微核;致突变性 Different hair coloring agent on Vicia faba root tip cell micronucleus effects Abstract: Along with the people living standard and beauty requirements continue to carry, high, the increasingly common use of hair coloring agent. As peoples living standards and aesthetic requirements continue to increase, the increasingly common use of hair coloring agent. These dyes have mutagenic, teratogenic, carcinogenic effects, has become a growing concern. Vicia faba root tip cell micronucleus technology is a kind of chromosomal damage and spindle toxicity as endpoints plant body cell detection method, because of its material culture, convenient, simple, rapid, accurate technology easy to master, has obvious dose - effect has become the current research and application of more detection environmental pollutant detection system [13]. In order to give hair coloring agent on the manufacture and management to provide a scientific basis, we choose the selling some hair coloring agent, using Vicia faba root tip cell micronucleus test. Determination of Vicia faba root tip cell micronucleus rate in this analysis, hair coloring agent mutagenicity, in order to determine the genetic material is toxic effect. We use a different hair coloring agent treatment of root tip cells of Vicia faba micronucleus rate per thousand, statistics ( micronucleus cell rate ) key words: Hair dye; Broad bean root point; Micronucleus; Mutagenicity 细胞的染色体发生断裂后,细胞进入下一次分裂时,染色体片段不能随有丝分裂进入子细胞,而在细胞质中形成直径小于主核的,嗜色与主核一致,完全与主核分开的圆形或椭圆形核,常福聚,赵庆夏.蚕豆根尖细胞微核试验[J]. 癌变,畸变,突变.遗传,1989,11(2):7-8. [5] /view/ae8e9bfe04a1b0717fd5dd66