小鼠接种HBV外膜小S蛋白产生的细胞免疫反应_药学论文.docVIP

小鼠接种HBV外膜小S蛋白产生的细胞免疫反应_药学论文 小鼠接种HBV外膜小S蛋白产生的细胞免疫反应_药学论文 【摘要】 目的 研究接种基因重组乙肝病毒疫苗RHBs产生的细胞免疫反应。方法 四组小鼠，腹腔分别接种0、0.65、1.25、2.5、5μg RHBs；4周后分离脾T淋巴细胞，分别检测CTL （cytotoxic T lymphocyte, CTL）及特异性增殖（T lymphocyte proliferation）反应。结果 CTL特异性释放率分别为：42.36%±19.32%、91.21%±22.97%、69.25%±24.13%、51.49%±21.661%，均高于0组小鼠CTL释放率31.21%±9.23% （对照），差异均有显著性（0.05）；0.65、1.25、2.5、5μg组小鼠特异性增殖刺激指数SI（体外刺激实验组cpm值/体外未刺激对照组cpm值分别为1.61、2.05、3.74、3.62；用精确Fisher方法分析每个小鼠CTL反应与T淋巴细胞特异性增殖反应的相关性，χ2=5.6979, 0.05，明显相关。结论 接种RHBs产生特异性CTL及T淋巴细胞增殖反应。 【关键词】 基因重组乙肝病毒疫苗；小鼠；接种；CTL；淋巴细胞特异性增殖 【Abstract】 Objective Aims the induction of hepatitis B virus （HBV） specific cellular immune response was studied in mice immunizated with recombinant HBV vaccine （RHBs）. Methods BALB/c（H-2d） mice were inoculated intraperitoneally（i.p.）with RHBs at the doses ranging from 0～5μg,4 weeks after the boosting immunization, spleen T lymphocyte were restimulated in vitro with specific peptides or RHBs separately,to detect specific cytotoxic T lymphocyte （CTL） response and specific helper T lymphocyte （HTL） proliferative activity. Results For CTL response, the percentages of specific release of the spleen lymphocyte in group 0.65、1.25、2.5、5 （mean±standard deviation） were 42.36%±19.32%、91.21%±22.97%、69.25%±24.13%、51.49%±21.661%, which were all greater than that in control group（31.21%±9.23%, 0.05）.The HTL proliferative activity is strongly associated with the CTL response in the mice immunization with RHBs （χ2=5.6979, 0.05 by exact Fisher test）. Conclusion Optimal HBV specific CTL response and HTL proliferative activity were induced for the mice immunized with RHBs. There were strong association between CTL response and HTL proliferative activity. 【Key words】 gene recombinant HBV vaccine （RHBs）；mice；inoculated ；CTL；lymphocyte spectific proliferation 乙肝病毒（Hepatitis B Virus, HBV）感染是一种危害人类健康的常见病、多发病，全世界HBV感染者有3亿人。接种HBV疫苗产生的特异性体液免疫反应对预防HBV感染有重要意义，而特异性细胞免疫反应在清除体内已有的HBV、治疗HBV感染过程中起着重要作用。 本文给BALB/c小鼠免疫接种不同剂量的HBV外膜小S蛋白（RHBs）疫苗，研究能否产生特异性细胞免疫反应、哪种剂量产生