炙甘草汤对扩张型心肌病SD大鼠心功能影响的实验研究_药学论文.docVIP

炙甘草汤对扩张型心肌病SD大鼠心功能影响的实验研究_药学论文 炙甘草汤对扩张型心肌病SD大鼠心功能影响的实验研究_药学论文 【摘要】 目的观察炙甘草汤对扩张型心肌病（DCM）SD大鼠模型心功能的影响并初步探讨其作用机制。方法 出生4周的雌性SD大鼠给予多柔比星建立扩张型心肌病（DCM）SD大鼠模型，给予炙甘草汤两周后观察实验前后大鼠超声心动图左室收缩末期容积（LVES）、舒张末期容积（LVED）、左室收缩末期内径（SD）、左室舒张末期内径（DD）、左室内径缩短率（FS）、左室射血分数（LVEF）和体重（BW）、心率（HR）、血浆神经体液因子的变化和大鼠心肌组织病理学的改变。结果经炙甘草汤治疗后的扩张型心肌病（DCM）SD大鼠超声心动图提示与实验前相比，LVEF、HR均明显增加（0.01），LVES明显降低（0.01），LVED，DD，SD较实验前有显著性差异（0.05）；血浆AngⅡ，ET，ANP较实验前有显著性差异（0.05），组织病理学检查实验组大鼠心肌病变减轻。结论 实验证明给予炙甘草汤治疗扩张型心肌病（DCM）SD大鼠可改善心功能，抑制RAAS系统的过度激活，降低动物死亡率，对扩张型心肌病有明显治疗作用。 　 【关键词】 炙甘草汤； 扩张型心肌病 动物模型 血浆神经体液因子 多柔比星 超声心动图　　Abstract：ObjectiveTo study the effects of ZGC on cardiac function in experimental animal model of dilated cardiomyopathy induced by adriamycin in SD rats and explore its possible mechanism. MethodsThe SD rat model of dilated cardiomyopathy were fed ZGC for two weeks which was established at a dose of 2mg/kg once a week by Adriamycin intraperitoneally injected. In order to evaluate the effects of ZGC on SD rat models of dilated cardiomyopathy, transthoracic echocardiography was performed in the experimental group SD rats after 2 weeks therapy of feeding ZGC to measure left ventricular dimensions and calculate ejection fraction. 2 weeks later, left ventricular slices of animal model were processed for histologica staining.ResultsCompared with the control group , LVEF、BW、HR increased significantly（0.01）and LVED、DD、SD were smaller and FS were larger than those of control（0.05）, whereas LVES in DCM rats of feeding ZGC decreased sharply（0.01）by transthoracic echocardiography measurement.The levels of serum angiotensin(AngⅡ),serum endothelin(ET)and serum atrial natriuretic peptide(ANP) were lower than those of control. The cardiac myocyte hypertrophy,degeneration and fibrosis were relieved accompanied with therapy of ZGC.ConclusionZGC has therapeutic effect on animal model of dilated cardiomyopathy and thus offers considerable newer therpeutic technologies. 　　Key words：ZGC; Dilated cardiomyopathy; Serum neurohormonal; An