碱性成纤维细胞生长因子及内皮细胞生长因子对兔韧带细胞增殖的影响_医学论文.docVIP

碱性成纤维细胞生长因子及内皮细胞生长因子对兔韧带细胞增殖的影响_医学论文 碱性成纤维细胞生长因子及内皮细胞生长因子对兔韧带细胞增殖的影响_医学论文 【摘要】 目的 观察碱性成纤维细胞生长因子(bFGF)和内皮细胞生长因子(ECGF)对内侧副韧带(MCL)和前十字韧带(ACL)细胞增殖行为的影响。方法 培养10周龄新西兰白兔内侧副韧带和前十字韧带细胞，在培养液中分别加入bFGF和ECGF，以XTT方法测定细胞的增殖行为。 结果 bFGF在1 ng/ml时即对MCL细胞有显著的促增殖作用，5 ng/ml时开始对ACL细胞有促进作用，其浓度达50 ng/ml时，对两种细胞的促进作用最大。ECGF在3.125 ng/ml时即对MCL细胞有显著的促增殖作用，其浓度达12.5 ng/ml时对ACL细胞有促进作用。bFGF和ECGF在超过其最佳作用浓度后，随浓度升高促进作用不再增强。结论 bFGF和 ECGF可以促进韧带细胞增殖进而促进韧带创伤愈合。 【关键词】 碱性成纤维细胞生长因子内皮细胞生长因子韧带细胞增殖 　　【Abstract】 Objective To observe the effects of basic fibroblast growth factor (bFGF) and endothelial cell growth factor (ECGF) on the proliferation of the cells from medial collateral ligament (MCL) and anterior cruciate ligament (ACL).Medthods The MCL and ACL cells of tenweekold skeletally immature New Zealand white rabbit were cultured, while bFGF and ECGF were added to the cell culture media, the cellular proliferation was assayed by XTT method.Results The addition of 1 ng/ml bFGF enhanced the proliferation of MCL cells significantly, 5 ng/ml bFGF enhanced the proliferation of ACL cells ,this enhancement of proliferation of both MCL cells and ACL cells was maximal in the concentration of 50 ng/ml. The adition of 3.125 ng/ml ECGF enhanced the proliferation of MCL cells significantly, 12.5 ng/ml ECGF enhanced the proliferation of ACL cells.The efficacy of bFGF and ECGF declined gradually when they present at concentrations greater than the dose with the greatest efficacy.Conlusion It is evident that bFGF and ECGF can enhance the proliferation of the ligamentous cells and they can enhance the healing of ligamentous issue injuries. 　　【Key words】 bFGF, ligament,proliferation,ECGF 　　韧带损伤是膝部的常见运动创伤，尤以内侧副韧带(medial collateral ligament,MCL)和前十字韧带(anterior cruciate ligament,ACL)多见，MCL和ACL是维持膝关节稳定的重要韧带，其损伤可使膝关节丧失稳定性，引起功能障碍，甚至导致骨关节炎[21]。在中央实质部完全断裂后，MCL可自然愈合，有时不需手术介入而ACL损伤后，即使手术缝合断端，其愈合率也较低。韧带损伤后的愈合过程与一般结缔组织相似，都经过急性炎症期、修复再生期和重塑或成熟期三个阶段[2]。研究表明[3]，在损伤韧带的局部存在着多种生长因子，它们被认为是韧带正常愈合过程的重要调节者。使用生长因子促进韧带愈合是近年来研究的焦点之一。作者通过体外培养MCL和