应用微卫星DNA技术研究中华蜜蜂群内工蜂监督效果.pdfVIP

中国农业科学 2008,41(6):1816-1821 Scientia Agricultura Sinica doi: 10.3864/j.issn.0578-1752.2008.06.032 应用微卫星DNA技术研究中华蜜蜂群内工蜂监督效果 1,2 3 1 1 1 谢宪兵 ，苏松坤 ，郑云林 ，吴小波 ，曾志将 1 2 3 （江西农业大学蜜蜂研究所，南昌 330045 ； 泉州师范学院，福建泉州 362000 ； 浙江大学动物科学院，杭州 310029 ） 摘要：【目的】研究不同中华蜜蜂（Apis cerana cerana ）群内的工蜂繁殖现象，探讨蜂群的工蜂监督效果。 【方法】以中华蜜蜂为试验材料，对处女蜂王分别进行单雄和双雄人工授精，并以蜂王自然交尾的蜂群作为对照。 蜂王成功繁殖后 7 周，利用蜜蜂微卫星 DNA 技术检测蜂群内的雄蜂是由蜂王产的未受精卵发育而成，还是由工蜂 产的未受精卵发育而成。【结果】所有蜂群中的雄蜂都是由蜂王产的未受精卵发育而成。【结论】在人工授精和自 然交尾蜂群中都明显存在工蜂监督行为。 关键词：中华蜜蜂；工蜂繁殖；工蜂监督；微卫星DNA；人工授精 Study on the Worker Policing in Apis Cerana Cerana Based on Microsatellite DNA 1,2 3 1 1 1 XIE Xian-bing , SU Song-kun , ZHENG Yun-lin , WU Xiao-bo , ZENG Zhi-jiang 1 2 ( Honeybee Research Institute, Jiangxi Agricultural University, Nanchang 330045; College of Quanzhou Normal, Quanzhou 362000, 3 Fujian ; College of Animal Sciences, Zhejiang University, Hangzhou 310029) Abstract: 【Objective 】 For exploring the effect of worker policing in Apis cerana cerana , the worker reproduction was studied in different colonies. 【Method 】 Biology of the Apis cerana cerana was the studied subject in this paper. Virgin queens were instrumentally inseminated with 1 or 2 males, and the controlled ones were naturally mated. Seven weeks later, the queens reproduced successfully, drones were detected whether developed from the