影响火鹤愈伤组织诱导、增殖和芽分化的因素研究.pdfVIP

Hans Journal of Agricultural Sciences 农业科学, 2012, 2, 43-48 doi:10.4236/hjas.2012.23008 Published Online September 2012 (/journal/hjas.html) Study on Factors Influencing Callus Induction, Proliferation and Bud Differentiation of Anthurium scherzerinum 1 1 1 1 2 Baogui Du , Caihong Liang , Yanghui Li , Maosheng Yi , Zhisheng Zhang 1Guangzhou Flower Research Center, Guangzhou 2Guangdong Provincial Key Laboratory of Plant Molecular Breeding, South China Agricultural University, Guangzhou Email: baoguidu@126.com Received: May 28th st th , 2012; revised: Jun. 1 , 2012; accepted: Jun. 11 , 2012 Abstract: In order to provide reference to the mass seedling production, the factors influencing the callus induction, proliferation and bud differentiation of Anthurium scherzerinum were studied by using the steriled seedling of 3 varieties such as Graffity as materials. Result: The results showed that the callus inducing rates of the 3 varieties were not obvious. 6-BA is the main influencing factor in the process of callus induction and bud differentiation. The inducing rate by using 2,4-D only was low, 20.84%. In the process of bud dif- ferentiation, the differentiation of combination of 6-BA + NAA is higher than that of combination of 6-BA + 2,4-D. The inducing rate and browning rate of callus were not obvious between different explants, in which the inducing rate of petiole was the highest, 97.22%, followed by leaf, the induction rate of root tip was the lowest, 93.06%. The related technical system of tissue