磷脂酶高产菌株的筛选和其诱变研究.pdfVIP

河流主题“流域行业点源水污染控制技术”研讨会·论文集 磷脂酶高产菌株的筛选及其诱变研究+ 2’ 王金梅1，戴大章2，姜芳燕2，李春1 l新疆兵团化工绿色过程重点实验室，新疆石河子832000； 2北京理工大学生命科学与技术学院，北京100081 摘要：以磷脂为唯一碳源的限制性培养基从富油土样中筛选得到136株产磷脂酶的微生物，摇 瓶复筛获得一株磷脂酶活性较高的菌株，经16SrDNA分子鉴定该菌株属于无色杆菌属 (Achromobacter sp．)。紫外线诱变处理后磷脂酶活力提高到39U／mL，较出发菌株提高了 73．33％。经进一步紫外线．硫酸二乙酯(DES)复合诱变后，磷脂酶活力达到44U／mL，较原始 菌株提高了95．56％。 关键词：磷脂酶，微生物，筛选诱变 andmutationofthestrainsfor Screening high—yieldphospholipase 2+ Jinmei Chunl 1，Dai Wang Dazhang2,JiangFangyan2,Li 1 School andChemical ofChemistry Engineering,Shihezi China 2School Institute ofLifeScience,BeijingofTechnology,Beijing100081，China as carbon were sole source．136strainsscreenedfromtheoil-richsoil Abstract：Usingphospholipid strainwith wasobtained． high activity samples．Aftershakingflask代-screening．onephospholipase 16SrDNA identificationindicatedthatit tothe sequence belongsspecies Uv-induced increasedto was mutation，t11ephospholipaseactivity 39U／mL。which53．85％higher thanthatofthe strain．After mutation．the enzyme original UV．diethylsulfate(DES)complex increasedto was thanthe 44U／mL．which stain． activity 95．56％higheroriginal Keywords：Phospholipase；high—yieM；Screening；Mutation 磷脂酶是一类能够降解磷脂的脂肪酶，可广泛应用于科学研究、油脂精练、磷脂改性、