Biological background- Molecular Laboratory Techniques.pptVIP

Molecular Laboratory Techniques Hybridizing DNA Copying DNA Cutting DNA Probing DNA Hybridization Hybridization exploits a potent feature of the DNA duplex – the sequence complementarity of the two strands Strands can be separated (denatured) by heating Remarkably, DNA can reassemble with perfect fidelity from separated strands Polymerase Chain Reaction (PCR) PCR is used to amplify (copy) specific DNA sequences in a complex mixture when the ends of the sequence are known Source DNA is denatured into single strands Two synthetic oligonucleotides complementary to the 3’ ends of the segment of i