单核苷酸多态性检测方法的研究进展汪维鹏.pdfVIP

HERED ITAS ( Be ijni g ) 28( 1): 117~ 126, 2006 1, 2 2 1, 2 汪维鹏 , 倪坤仪 , 周国华 ( 1. , 210002; 2. , 210038) : ( single nucleoti e polym orphism, SNP) SNP SNP , SNP , , SNP : ; ; + : Q524 . 3 : A : 0253- 9772( 2006) 01- 0117- 10 Approaches for SNP Genotyping 1, 2 2 1, 2 WANG W eiPeng , N IKunY i, ZHOU GuoHua ( 1. Hua o ng Re se a rch Ins titute fo r Me icine a n Bio te chnics, Nan jing 210002, China; 2. Depa rtm ent o fAna ly tica lChem istry, C hni a P ha rm a ce utica lUnive rsity, Nanjing 210038, C hni a ) Abstract:W ith the com pel tio of the Huma Ge ome Project ( HGP), typi g s i gle ucleotide polymorphisms ( SNPs) has become o e of the mai tasks i the postge ome era. Co seque tly, a robust, flexible a d cost effective tech ique for SNP typi g is esse tial to a alyze a al rge umber of SNPs. The al test ge otypi g tech ologei s a d the relative detectio platforms were i troduced systematically. The pri ciple of SNP typi g was descrbi ed i detail i respect of alleel specific hybrdi izatio , restrictio e zyme digestio , prmi er exte s oi a d oligo uc el otdi e ligatio assay, aswe ll as the ge otypi g platforms of plate readers, ge echpi s, bead array a d mass spectrometry. Moreover, the w ay to the highthroughput ge otypi g i the future was brei fly discussed. Key wor s: SNP; ge otypi g tech ologies; ge otypi g platforms 5 ( single nucleoti e polymor , 10 SNP