中国南海印马刺尖柳珊瑚Echinomuricea+indomalaccen化学成分研究.pdfVIP

中国南海印马刺尖柳珊瑚 Echinomuricea indomalaccensis 化学成分的研究 曹飞，邵长伦，王长云∗ (中国海洋大学医药学院，海洋药物教育部重点实验室，山东 青岛 266003) 摘 要：目的 对中国南海印马刺尖柳珊瑚（Echinomuricea indomalaccensis ）的甾醇类化学 成分进行研究，从中寻找具有抗菌活性的次级代谢产物。方法 采用多种色谱方法进行分离 纯化，通过理化性质和波谱数据鉴定化合物的结构；利用抗菌活性筛选模型，评价甾醇类化 合物的抗菌活性。结果 从乙酸乙酯相中分离得到8 个甾醇类化合物，其中 2 个为新化合物： indomasteroids A (1), indomasteroids B (2) ； 6 个 为 已 知 化 合 物 ： (22E)-cholest-22-ene-1β,3β,5α,6β-tetrol (3), 24-methylenecholestane-1β,3β,5α,6β-tetrol (4), (24S)-ergosta-1β,3β,5α,6β-tetrol (5), (22E,24S)-5α,8α-epidioxy-ergosta-6,22-dien-3β-ol (6), (24S)-5α,8α-epidioxy-ergosta-6-en-3β-ol (7), 5α,8α-epidioxy-cholesta-6-en-3β-ol (8) 。结论 化合 物 1, 3, 7 对枯草芽孢杆菌 (Bacillus subtilis) 和四联球菌 (Micrococcus tetragenus) 显示出 了一定的抑制活性。 关键词：印马刺尖柳珊瑚；Echinomuricea indomalaccensis ；化学成分；抗菌活性 Studies on Chemical Constituents of Gorgonian Echinomuricea indomalaccensis from the South China Sea CAO Fei, SHAO Chang-lun, WANG Chang-yun* (Key Laboratory of Marine Drugs, Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China) ABSTRACT: OBJECTIVE To study the sterols from gorgonian Echinomuricea indomalaccensis collected from the South China Sea to find antibacterial compounds. METHODS Chromatography was performed for the isolation and purification of the compounds, the structures were determined by extensive spectroscopic analysis in association with physical and chemical properties, as well as comparison of their spectral data with those reported in literatures. The bioactivities of the sterols were evaluated with antibacterial models. 基金项目：国家自然科学基金; 山东省自然科学基金(ZR2011DQ019)资助。 作者简介：曹飞 (198