粘质沙雷氏菌酶催化H2O2降解对苯二酚地研究.pdfVIP

粘质沙雷氏菌酶催化H2O2 降解对苯二酚的研究 孙 敏 姚日生* 黄辉 何红波 王春玲 （合肥工业大学化工学院，农产品生物化工教育部重点实验室，安徽合肥230009 ） 摘 要 利用源于粘质沙雷氏菌（Serratia marcescens）AB 90027 的酶催化H O 降解对苯 2 2 二酚，借助分光光度法对 H O 用量、温度、pH 及振摇速率等降解反应条件进行了研究，测 2 2 定了催化降解前后溶液的CODCr 值，采用UV-VIS 吸收光谱、IR 吸收光谱考察了降解前后分 子结构的变化。结果表明，粘质沙雷氏菌酶催化H O 可降解对苯二酚并将其开环，该体系降 2 2 解的最佳条件为每降解 100mg 对苯二酚H O 用量5mL、温度40℃、pH9.5，降解后对苯二酚 2 2 CODCr 去除率可达96.3％。 关键词 粘质沙雷氏菌；酶；双氧水；对苯二酚；降解 DEGRADATION OF HYDROQUINONE BY HYDROGEN PEROXIDE OXIDATION USING ENZYME FROM Serratia marcescens AS CATALYZER SUN Min, YAO Risheng*, HUANG Hui, HE Hongbo and WANG Chunling （Key Laboratory of Bio·Process, Hefei University of Technology ,M inistry of Education, P.R.C,, Hefei 230009, Anhui, China ） Abstract This paper reports on the degradation of hydroquinone using hydrogen peroxide as oxidizer and enzyme from Serratia marcescens AB 90027 as catalyzer. The effecting factors such as volume of H O , temperature, pH and shaking speed were studied by ultraviolet 2 2 spectrophotometry. CODCr before and after degradation were measured according to standard method. Variation of molecular structure during degradation was investigated by UV-VIS spectrum and IR spectra. The results showed that the enzyme from Serratia marcescens AB 90027 could catalyze hydrogen peroxide to oxidize hydroquinone and break its phenyl ring. In the degradation, the optimal volume of H2O2 , temperature and pH were 5mL(per 100mg hydroquinone) , 40 ℃and 9.5, respectively. And the CODCr removal efficiency could get to 96.3 ％. Keywords Se