电感耦合等离子体原子发射光谱测定葡萄糖酸锌中8种元素.docVIP

ICP-AES法测定葡萄糖酸锌中8种元素 闫清华1, 杨理2 新乡医学院生命科学技术系，河南 新乡 453003；2. 河南科技学院实验中心，河南 新乡 453003） 摘 要：用模拟胃酸和微波消解法对葡萄糖酸锌进行消化，以电感耦合等离子体发射光谱仪 ICP-AES 检测葡萄糖酸锌中Cu、Fe、Zn、Mn、Na、K、Mg、Ni八种金属元素的含量，并优化微波处理条件。结果表明：ICP-AES检测各元素的相对标准偏差在1.03%～3.37%之间，各元素回收率在96.32%～108.56%之间。两种方法对8种待测元素的消化效果基本相同, 从另一角度说明人体对葡萄糖酸锌有较好的吸收。采用微波消解技术和ICP-AES相结合检测重金属元素具有省时、省力、环境污染小，测定结果快速、准确等优点。葡萄糖酸锌中图分类号：O657.31 文献标识码：A Determination of Eight Elements in Zinc Gluconate by ICP-AES YAN Qing-hua 1 , YANG Li 2 1. Department of Life Science and Technology, Xinxiang Medical College, Xinxiang Henan 453003; 2. Department of Experimental Center, Henan Institute of Science and Technology, Xinxiang Henan 453003 Abstract：The contents of eight metal elements Cu、Fe、Zn、Mn、Na、K、Mg and Ni in zinc gluconate were studied by ICP-AES with the stomach acid simulated and microwave digestion, The optical condition of microwave digestion measurement were optimized. The results showed that relative standard deviations and recoveries of all elements were determined with ICP-AES between1.03%～3.37% and 96.32%～108.56%, respectively.The digestive effects of eight elements were almost same in the above two methods, on the others hand, zinc gluconate could be possible completely absorbed by human body. Conbining the microwave digestion technology with the ICP-AES，There were a lot of merits of time-saving, reduces effort, small environmental pollution, fast and accurate determination result in determining heavy metal elements . Keywords：inc gluconate; Microwave digestion; ICP-AES 锌是人体所必需的微量元素之一, 体内有近200种酶的活性与锌有关, 如DNA聚合酶、RNA合成酶、碱性磷酸酶及多种还原酶等40多种酶为含锌酶。锌对于维持人体正常代谢发挥多种生理功能, 故被称为“生命之花”[1]。葡萄糖酸锌片是一种人工合成抗缺锌药物,无毒,由于它对胃肠道刺激比硫酸锌小，且口服利用率高是目前首选的补锌药物和强化剂。电感耦合等离子体原子发射光谱法 ICP-AES 灵敏度高、干扰少、线性范围宽并且能连续测定多种元素已成为现代分析测试技术中重要的检测手段[2,3]。本文采用模拟人体胃酸溶解和微波消解技术消解葡萄糖酸锌片并采用电感耦合等离子体原子发射光谱同时测定两种消化液中8种金属元素的溶出率，判断胃酸对各种金属元素的吸收情况，为人类对其他药物中金属元素的吸收利用及检测提供了一种简单、有效的分析方法。 验部分 .1 仪器与Optima 2100 DV电感耦合等离子体发射光谱仪MAS微波消解仪TDL-5A型台式低速离心机TJ-EHP 1000电加热板，梅特勒-托利多S1 000 mg·L-1 Cu、Fe、Zn、Mn、Na、