近红外漫反射光谱法鉴别不同厂家注射用头孢他啶.pdfVIP

近红外漫反射光谱法鉴别不同厂家注射用头孢他啶 1 1 1 2 潘颖 沈漪 项竞佐 刘全 1 上海市药品检验所抗生素室 200233 2 美国 Thermo Nicolet 仪器公司 摘要：采用近红外漫反射光谱法对不同厂家注射用头孢他啶进行快速无损地鉴别分析。在不打开包装瓶 的条件下，对来源于 7 个不同厂家和不同批次的 61 个样品分别采集了 3 次近红外漫反射光谱，并以其 中 120 条光谱组成校正集，用于建立判别分析模型，以其余 63 条光谱组成验证集，以检验判别模型的 判别效果和准确性。结果表明，所建立的判别模型对验证集中的样品作出能够准确无误地判别分类。该 方法方便快速、准确有效，对打击市场上假药、劣药具有重要意义。 关键词：近红外（NIR）漫反射光谱；注射用头孢他啶；判别分析 Identification of the Ceftazidime for injection by NIR Diffuse Reflectance Spectroscopy Pan ying1 1 1 2 , Shen yi , Xiang jingzuo , Liuquan (1. Shanghai Institute for Drug Control,Shanghai 200233) (2. Thermo Nicolet corporation, U.S.A) Abstract To fast and nondestructively identify the Ceftazidime for injection by NIR diffuse reflectance spectroscopy. 61 samples from different batches of 7 factories were prepared and three spectra were collected of each sample. 120 out of all 183 spectra were used to form the calibration set to build the discrimination model, and the other 63 spectra were used to validate the performance of the model. The results show that the model can distinguish all samples from 7 different factories actually. The presented method is convenient, fast, and have a good performance. It is very important to beat the false and poor drugs on the market. Key words Near Infrared Diffuse Reflectance Spectroscopy, Ceftazidime for injection, Discriminant analysis 近红外（Near Infrared ，NIR ）光谱是指界于可见光与中红外光之间的电磁波，波长范围是 780～ 2500nm （12000～4000cm-1 ），的主要谱峰为分子中C-H、N-H 、O-H等含氢基团的倍频吸收和合频吸收， ［ ］ 因此NIR光谱分析技术适合与这些基团有直接或间接关系的有效成分的定量和定性分析 1