银荆感冒颗粒抗乙型流感病毒的药效学研究_药学论文.docVIP

银荆感冒颗粒抗乙型流感病毒的药效学研究_药学论文 银荆感冒颗粒抗乙型流感病毒的药效学研究_药学论文 作者：邱召娟，朱萱萱，倪文澎，严士海，朱琼 【摘要】 　　目的研究银荆感冒颗粒对乙型流感病毒（B/京防98-96）的抑制作用。方法体外接种乙型流感病毒鼠肺适应株于狗肾细胞(MDCK)，观察银荆感冒颗粒体外抗病毒作用；体内实验①以乙型流感病毒鼠肺适应株滴鼻感染小鼠，观察14 d，计算小鼠存活率及存活天数；体内实验②以乙型流感病毒鼠肺适应株滴鼻感染小鼠，观察肺指数。结果银荆感冒颗粒体外抗乙型流感病毒EC50为3.68 mg/ml。银荆感冒颗粒10 g/kg和20 g/kg剂量组可明显延长乙型流感病毒感染小鼠存活天数，降低死亡率；银荆感冒颗粒20 g/kg剂量组可明显抑制肺指数值，抑制率为36.7%。结论 银荆感冒颗粒对乙型流感病毒有一定的抑制作用。 【关键词】 银荆感冒颗粒 乙型流感病毒 体内外 抑制 　　Abstract：ObjectiveTo investigate the inhibitory effect of Yinjingganmao granule on Influenza B Virus. Methods The inhibitory cytopathic effects(CPE) of Yinjingganmao granule were observed by MDCK cell culture. The experiment in vivo were done by the following two methods：(1) The mice were given Yinjingganmao granule for 7 days and 30 μl Influenza B Virus by nose at sequenti luce. We calculated the survival rate and days of mice 14 days later. (2) The mice were given intragastrically Yinjingganmao granule for 7 days and 30 μl Influenza B Virus by nose at sequenti luce. We calculated the lung index and observed the pathological change. ResultsThe EC50 of Yinjingganmao granule on Influenza B Virus was 3.68mg/ml. The 10g/kg and 20 g/kg groups of Yinjingganmao granule could prolong the survival days and reduce the death rate significantly. The 10g/kg and 20 g/kg groups of Yinjingganmao granule could inhibit the lung index of the infected mice and the inhibition ratio was 31.4 and 29.1% respectively. In addition, it could also lessen the pathological change.Conclusion：Yinjingganmao granule has inhibitory effect on Influenza B Virus. 　　Key wordsYinjingganmao granule; Influenza B Virus; In vivo and vitro; Inhibition 　　流行性感冒是上呼吸道传染性极强的病毒感染性疾病，在呼吸系统中发病率占首位，可引起许多并发症，重者可引起死亡。我们运用现代工艺，选用古方银翘散结合临床化裁而制成银荆感冒颗粒，作为本院院内制剂，在临床上取得了良好疗效。为了探索该颗粒剂治疗流行性感冒的机制，我们选择与呼吸道有关的乙型流感病毒，以该病毒感染小鼠为药物评价模型，通过感染小鼠存活率、存活天数、肺指数值与肺病理变化为指标来评价药效，同时结合体外实验，来观察银荆感冒颗粒对乙型流感病毒引起的细胞病变的作用。 　　1 材料与仪器 　　1.1 药物及试剂银荆感冒颗粒，江苏省中医院药剂科提供，批号050424；利巴韦林注射液，四川美大康药业股份有限公司，批号050316；RPMI Medium 1640，GIBCO公司；胰蛋白酶，上海伯奥生物技术有限公司，批号020602；