银杏叶提取物对MPP+诱导的中脑多巴胺能神经细胞损伤的保护作用_医学论文.docVIP

银杏叶提取物对MPP+诱导的中脑多巴胺能神经细胞损伤的保护作用_医学论文 银杏叶提取物对MPP+诱导的中脑多巴胺能神经细胞损伤的保护作用_医学论文 作者：奚淑芳,陈建宗,王百忍,杨浩,高建苑,田季雨,康小刚 【关键词】 帕金森病 Protective effect of EGB761 on embryonic rat mesencephalic dopaminergic neurons in vitro from MPP+induced injury 　　【Abstract】 AIM: To explore whether the ginkgo biloba extract (EGB761) could partially protect embryonic rat mesencephalic dopaminergic (DAergic) neurons from MPP+induced injury. METHODS: Primarily cultured cells were dissociated from embryonic rat mesencephalon and the cells were treated by addition of EGB761 and MPP+. The viability of mesencephalic primary cells and DAergic neurons was assessed by MTT assay and the survival number of tyrosine hydroxylase (TH) positive cells was evaluated by immunocytochemistry. The level of nitric oxide (NO) was assessed by immunocytochemistry for inducible nitric oxide synthase (iNOS). RESULTS: The number of THpositive cells and the viability of mesencephalic cells significantly decreased while the number of iNOS cells increased in the development of apoptosis induced by MPP+. The adding of EGB761 significantly reversed these changes caused by MPP+. CONCLUSION: EGB761 can protect embryonic rat mesencephalic DAergic neurons against MPP+induced apoptotic death in a dosedependent manner. 　　【Keywords】 ginkgo biloba plant extract mesencephalic cells, cultured Parkinsons disease 1methyl4phenylpyridiniumion 　　【摘要】 目的：探讨银杏叶提取物（EGB761）对1甲基4苯基吡啶离子（MPP+）诱导的帕金森病细胞模型是否具有保护作用. 方法：采用胚胎大鼠中脑原代细胞培养法，分离培养中脑神经细胞，随后进行EGB761及MPP+处理，最后通过3(4,5二甲基噻唑2)2,5二苯基四氮唑溴盐（MTT）微量比色法，酪氨酸羟化酶（TH）免疫细胞化学，研究EGB761对MPP+损伤后的中脑原代培养细胞及DA能神经细胞活性的影响，同时进行iNOS免疫细胞化学法，了解iNOS的表达情况. 结果：在MPP+诱导的中脑原代细胞凋亡中，MPP+组TH阳性细胞数及细胞存活率均显著低于各EGB761组及阳性对照组(0.01). 而iNOS的表达则显著多于各EGB761组（0.01）. 结论：EGB761对MPP+诱导的胚胎大鼠中脑原代培养细胞损伤具有保护作用，且此作用有随剂量的增大而增加的趋势. 　　【关键词】 银杏叶植物；提取物；中脑；细胞，培养的； 帕金森病； 1甲基4苯基吡啶离子 　　0引言 　　帕金森病（Parkinsons disease, PD）是以中脑黑质致密带多巴胺（dopamine, DA）能神经元变性、脱失为主要特征的神经系统变性疾病. 其机制可能与黑质纹状体氧化应激反应增强和局部抗氧化保护机制减弱有关. 目前，PD的治疗仍以症状治疗为主，还没有一种治疗能够延缓其病情的进展. 最近的研究发现，银杏叶提取物(EGB761)具