髓内循环破坏致骨内高压兔模型的建立及减压的影响_医学论文.docVIP

髓内循环破坏致骨内高压兔模型的建立及减压的影响_医学论文 髓内循环破坏致骨内高压兔模型的建立及减压的影响_医学论文 【摘要】 目的： 建立一种髓内循环破坏致骨内高压形成的动物模型的可靠方法并观察钻孔减压的影响. 方法： 采取兔单侧股骨髓腔内灌注骨水泥，破坏股骨髓内循环致骨内高压形成. 分别测量钻孔减压组和未钻孔减压组不同时段髓内压的改变，通过HE染色光镜、电子显微镜以及免疫组化等方法，观察不同时段远端关节软骨的形态和代谢改变. 结果： 破坏髓内循环后骨内高压形成，在未钻孔减压组骨内高压长时间存在，随时间推移压力呈下降趋势，但32 wk后仍高于正常，远端关节软骨出现骨性关节炎代谢表现；在钻孔减压组，一次钻孔后，骨内压就可恢复正常水平，未见关节软骨损害出现. 结论： 所建立的髓内循环破坏致骨内高压形成的动物模型可靠, 持续时间较长且远端关节软骨出现相应损害；钻孔减压对治疗骨内高压简单有效. 【关键词】 髓内循环；骨内高压；钻孔；减压；软骨,关节；代谢 　　【Abstract】AIM: To develop a reliable method of animal modeling of high intraosseous pressure (IP) resulted from the damage of intraosseous circulation system and observe the effect of drilling decompression treatments. METHODS: The rabbit model was established by blocking proximal and middle femoral medullary cavity of one side with PMMA to damage intramedullary blood circulation and to cause continuous high IP. IP between drilling decompression group and undrilling decompression group was compared at different time points. HE staining, light microscope, eletron microscope and immunohistochemical method were used to observe the morphology of distal articular cartilage and its metabolism changes at different time points. RESULTS: High IP was formed after the damage of the intramedullary blood circulation and it lasted for a long time in undrilling decompression group.With time going on, the pressure showed an obvious trend of decline, but it was still higher than the normal level after a period of 32 weeks distal articular cartilage presented osteoarthritis and metabolism. As for the drilling decompression group, the IP almost recovered to its normal level without showing damage of articular damage. CONCLUSION: A reliable animal model of high IP resulted from the damage of the intraosseous circulation system can be established successfully.High IP can last for a long time with damage of distal articular cartilage. Drilling decompression is simple and effective to treat high IP. 　　【Keywords】intramedullary blood circulation high intraosseous pr