RNAi技术在抗葡萄病毒A的初探.pdfVIP

RNAi技术在抗葡萄病毒A的初探 王建辉也，刘建军铲，陈克玲1，一，李洪雯1’2 (1四川省农业科学院园艺研究所，成都610066：2农业部西南地区园艺作物生物学与种质创制重点实验室，成都 610066：3四川省农业科学院成都610066) Hrus 摘要：使用Clones A,GVA)分离物中的139个病毒部 PCR-RFLP技术分析了15个葡萄病毒A(Grapevine 分区域的克隆序列，结果表明葡萄常常混合感染多种变异株。以34—I-4变异株的外壳蛋白基因构建RNAi载体 隆成功接种到本氏烟草(Nicotiana 基础。 resistanceto StudiesontheNicotianabenthamianaconferred Preliminary VirusA RNAi Grapevineby WANG Jian-hui地，LIU Jian-junr，CHENKe．1ingL2，LIHong-wen2 Institute,Sichutm (。Horticulture AcademyofAgriculturalSciences,Chengdu610066,China；。铆 horticultural and enhancement加southwest laboratoryof cropsbiologygermplasm regions,ministryof 610066,China；’SichuanAcademy 610066,China) agriculture,Chengdu ofAgriculturalSciences,Chengdu variants Virus identifiedrestriction Abstract：The of divergent GrapevineA(GVA)wereby fragment of chainreactionof clonedfrom lengthpelymorphismpolymerase plasmids amplifiedproducts(clone 139 from15GVAisolates．Thefull ofcoat ofvariants PCR-RFLP)inplasmids length proteingene 3-4-4-4wasconstructedinto structurein 121-dsCPandtransformedinto hairpin pBI competent infectiouscloneofIsl51GVAisolateWas inoculated GV3103．Besides，the agrobacterium successfully intotheleavesofNicotiana achievementscouldtO Oil benthamiana．Therefore，these study help acquired GVA-resistanceinthemodel RNAiinfuture． plantby 葡萄是