不同碳氮组合对三角褐指藻兼养生长及脂质积累的影响.pdfVIP

2014年第39卷第5期摇 摇 摇 摇 摇 摇 摇 摇 摇 摇 摇 摇 摇 中摇 国摇 油摇 脂 57 微生物油脂 不同碳氮组合对三角褐指藻兼养生长及 脂质积累的影响 宋雅琪,周启霞,吴摇 琼,何凌峰,朱静静,王海英 (中南民族大学微生物与生物转化重点实验室,武汉430074) 摘要:为了探讨兼养培养中不同碳氮组合对三角褐指藻(P. tricornutum)生长及脂质积累的影响, 以葡萄糖为碳源,氯化铵为氮源,以不同的碳氮组合兼养培养P.tricornutum,用干重法测定P.tricor鄄 nutum 的生物量,三氯甲烷-甲醇法提取脂质,并采用GC分析脂肪酸组成。 结果表明,兼养生长中 P.tricornutum基于氯化铵消耗的细胞得率系数随着葡萄糖质量浓度的增加而降低,降低了对氮源 的需求;在碳氮组合为葡萄糖质量浓度0.5 g/ L,氯化铵浓度 1.0 mmol/ L 的条件下,P.tricornutum 生物量达最高,为1.25 g/ L,脂质含量可达44.6%,不同碳氮组合对P.tricornutum 脂质多不饱和脂 肪酸(PUFA)和二十碳五烯酸(EPA)含量的影响不显著。 关键词:三角褐指藻;生物量;脂质积累;氯化铵;葡萄糖 中图分类号:TS222;Q936摇 摇 摇 文献标志码:A摇 摇 摇 文章编号:1003-7969(2014)05-0057 -04 Effect of combination of carbon and nitrogen on growth and lipid accumulation ofP. tricornutum under mixotrophic cultivation SONG Yaqi,ZHOU Qixia,WU Qiong,HE Lingfeng, ZHU Jingjing,WANG Haiying (Key Labfor Microorganismsand Biotransformation,South-Central University for Nationalities,Wuhan430074,China) Abstract:The effects of different combinationsof carbon andnitrogen sourcesonthegrowth andlipidac鄄 cumulation of P. tricornutum under mixotrophic cultivation were studied. The carbon sourcewas glucose and the nitrogen source was ammonium chloride. The biomass of P. tricornutum was determined by dry weight method. The lipid was extracted by chloroform -methanol and the fatty acid composition of the lipid was analyzedby GC. Theresultsshowedthat the cellyield coefficient based on consumption of am鄄 monium chloride reduced with the mass concentration of glucose increasing,so the demand for nitrogen source dropped;under the conditions of mass concentration of glucose0.5 g/ L and concentration of am鄄 monium chloride 1.0 mmol/ L,the biomass and lipid content of P. tricornutum reached 1.25 g/ L and 44.6% r