在大剂量5氟尿嘧啶治愈荷瘤小鼠过程中外周血细胞的变化与肿瘤消退之间的相关性分析_临床医学论文.docVIP

在大剂量5氟尿嘧啶治愈荷瘤小鼠过程中外周血细胞的变化与肿瘤消退之间的相关性分析_临床医学论文 在大剂量5氟尿嘧啶治愈荷瘤小鼠过程中外周血细胞的变化与肿瘤消退之间的相关性分析_临床医学论文 作者：李传刚, 李墨林*, 舒晓宏, 刘用楫, Michael Notter, 秦志海 【摘要】 　　目的: 观察在单一剂量5氟尿嘧啶(5治愈荷瘤小鼠过程中外周血细胞数量的变化与肿瘤结节缩小之间的关系, 为进一步研究化疗治愈肿瘤的作用机制奠定基础。方法: 利用单一剂量5治愈荷瘤野生型C57BL/6小鼠模型, 荷瘤小鼠腹腔内分别注入25、 75、 135 mg/kg的5等量生理盐水对照, 确定5治愈肿瘤的量效关系。然后, 再取6只荷瘤小鼠, 分别在75 mg/kg 5治疗前3 d、 治疗后第1、 4、 7、 11、 15、 28天内眦静脉取血, 进行血常规检测, 分析外周血白细胞、 血红蛋白和血小板的变化与荷瘤小鼠肿瘤结节缩小之间的相关性。结果: 75、 135 mg/kg的5均可使荷瘤小鼠的肿瘤结节在治疗后1周内逐渐缩小、 直至消退, 因此5治愈荷瘤小鼠的最低有效量为75mg/kg。75mg/kg 5治疗后第1天外周血白细胞降为(5.0±1.1)×109/L, 并持续到化疗后第4天, 与治疗前(9.5±1.58)×109/L比较差异有统计学意义(0.01); 5治疗后第7～15天外周血白细胞的数量升至化疗前水平, 随后逐渐下降, 在治疗后第28天降至（5.9±1.96）×109/L, 低于化疗前水平(0.01)。Hb含量在5治疗后第1天也出现降低, 由化疗前的(133±7) g/L降为(112±9) g/L, 差异有统计学意义(0.01), 随后Hb含量持续在低水平, 治疗后15 d 逐渐恢复至化疗前水平。75 mg/kg的5对荷瘤小鼠血小板的抑制作用不明显, 反而在治疗后第1、 11天出现2次血小板一过性增高(0.01)。结论: 75 mg/kg 5可引起外周血白细胞数和血红蛋白浓度的降低, 但对血小板的抑制作用不明显。5发挥抗肿瘤作用使荷瘤小鼠的肿瘤结节缩小与化疗后外周血白细胞和Hb的降低无关, 而与化疗后第1天血小板计数的增加有关。 【关键词】 5 氟尿嘧啶(5肿瘤治愈 淋巴瘤 C57BL/6小鼠 血小板计数 　　[Abstract] AIM: To investigate the relationship between the alterations of complete blood counts and tumor shrinkage during tumor rejection induced by a high dose of 5 was the minimal effective dose of 5FU that could cure the tumorbearing mice. Then another 6 tumorbearing mice were treated intraperitoneally with 5FU (75 mg/kg). Blood samples were obtained from orbital venous sinus on different days before and after 5FU treatment, and then complete blood counts were performed and the relationship between the alterations of blood counts and tumor shrinkage after 5FU treatment was analyzed. RESULTS: Tumor sizes decreased steadily and tumors disappeared within the first week after 5FU treatment; and at the same time 75 mg/kg 5FU also had side effects on peripherial blood cells. The number of WBC significantly decreased from the first day after 5FU treatment (0.001). But during the 7 th to 15 th day the number of WBC rebounced back to normal level (Pgt;0.05). Later it decreased again and it couldn’t recover bac