哇巴因对大鼠肾皮质Na+K+ATP酶活性及多巴胺D1受体mRNA表达的影响_临床医学论文.docVIP

哇巴因对大鼠肾皮质Na+酶活性及多巴胺D1受体mRNA表达的影响_临床医学论文 哇巴因对大鼠肾皮质Na+酶活性及多巴胺D1受体mRNA表达的影响_临床医学论文 作者：张玉蓉，袁祖贻，任延平 【摘要】 目的 观察哇巴因对大鼠肾皮质Na+酶活性及多巴胺D1受体mRNA表达的影响。方法 28只SD大鼠随机分为2组，即哇巴因组[27.8μg/(kg·d)哇巴因腹腔注射]和对照组(生理盐水1mL/d腹腔注射)，每周测量鼠尾动脉血压，共5周。于第3、5周后分2批处死动物，应用分光光度法测定各组大鼠肾皮质Na+酶活性，同时采用实时定量PCR(reale PCR)观察肾皮质中多巴胺D1受体mRNA表达的变化。结果 大鼠腹腔注射哇巴因3周后，两组血压无显著性差异，但哇巴因组大鼠肾皮质Na+酶活性高于对照组(0.01)，多巴胺D1受体mRNA表达低于对照组(0.01)。5周后，哇巴因组大鼠血压与对照组相比有显著性差异(0.01)，哇巴因组大鼠肾皮质Na+酶活性进一步增高(0.01)，多巴胺D1受体mRNA表达进一步减低(0.01)。结论 长期小剂量外周注射哇巴因可使SD大鼠血压持续升高，这一作用与肾近曲小管Na+酶活性增加引起水钠潴留有关，多巴胺D1受体可能参与了这一过程。 【关键词】 Na+酶活性;多巴胺D1受体;哇巴因;高血压;钠转运 　　Medical School of Xidopamine D1 receptor in rat kidney cortex. Methods A total of 28 male SpragueDawley (SD) rats were randomly divided into ouabain group and control group, which were treated with ouabain or saline for 5 weeks; rat tail systolic blood pressure (SBP) was recorded weekly. Rats were sacrificed after 3 and 5 weeks, respectively. Then Na+K+ATPase activity and the expression of dopamine D1 receptor in rat kidney cortex were measured by colorimetric assay and realtime PCR, respectively. Results After 3 weeks of ouabain treatment, the mean SBP did not change significantly, but the Na+K+ATPase activity increased (0.01) and the expression of dopamine D1 receptor decreased (0.01) in comparison with those in control group. After 5 weeks, the mean SBP was significantly higher in ouabain group than in control group (0.01); the Na+K+ATPase activity further increased (0.01) and the expression of dopamine D1 receptor further decreased (0.01). Conclusion Chronic peripheral administration of lowdose ouabain can constantly raise blood pressure of rats, which may be related to the increased renal sodium retention induced by the increased renal cortical Na+K+ATPase activity. Dopamine D1 receptor might be involved in this process. 　　KEY WORDS: Na+ne D1 receptor; ouabain; hypertension; sodium handling 　　肾脏是人体负责钠排泄的重要器官，其对水钠排泄的异常是高血压发病机制中的重要环节，其中肾脏近曲小管钠水重吸收增加是高血压形成的主要机制[1]。肾脏近曲小管上皮离子转运非常复杂。但是，近曲小管钠转运的