外源核苷酸对多代大鼠血清尿酸水平及肾脏损伤的影响_临床医学论文.docVIP

外源核苷酸对多代大鼠血清尿酸水平及肾脏损伤的影响_临床医学论文 外源核苷酸对多代大鼠血清尿酸水平及肾脏损伤的影响_临床医学论文 作者：马奕 徐琳琳 许雅君 李勇 【摘要】 目的 通过三代繁殖试验，观察外源性核苷酸对于啮齿类大鼠血清尿酸水平、肾脏损伤的影响。 方法 断乳大鼠(F0代)喂饲核苷酸含量分别为0、0.01 %、0.04 %、0.16 %、0.64 %、1.28 %的受试物饲料90 d后，雌、雄1∶1交配，所产仔鼠为Fl、Fl断乳后喂养90 d后进行二代繁殖试验，重复上述实验方法直到多代繁殖实验结束，F3代出生;每一代大鼠在断乳后90 d，留取血液样本进行血清尿酸、尿素氮、肌酐含量及肾脏病理检查。 结果 外源核苷酸使F1、F2、F3代雄性大鼠血清尿酸含量显著下降，对雌性大鼠无显著影响。4代大鼠均未见肾脏的病理改变。 结论 在本实验剂量下，补充外源性核苷酸可降低后代雄性大鼠血清尿酸含量，且未见肾脏损伤。 【关键词】 外源核苷酸;血清尿酸;肾脏损伤 　　【Abstract】 Objective To observe the effect of serum uric acid levels and renal function by exogenous nucleotides in multigeneration rats. Methods Weanling rats(F0) were fed to exogenous nucleotides at the dose of 0, 0.01 %, 0.04 %, 0.16 %, 0.64 %, 1.28 % for 90 days and then male and female rats mated for 1∶1 pattern. The offspring of F0 was F1. F1 rats were fed to exogenous nucleotides for 90 days after they were weaned. And then go on to the second reproductive experiment. Repeat the progress above until F3 rats were born. After every generation fed for 90 days, blood samples were collected to detect the contents of serum uric acid, urea nitrogen, creatinine, and then the pathologic examination of kidney was performed. Results Serum uric levels of F1,F2,F3 male rats in dose groups were significantly lower than those of control group, but the same result didn′t appear in female rats. No renal insufficiency and Pathological changes were found in the four generation rats. Conclusion At the dose of the experiment, supplementing exogenous nucleotides could decrease serum uric acid levels of male descendant rats and renal injury was not been found. 　　【Key words】 Exogenous nucleotides; Serum uric acid; Renal injury 　　近年来，外源核苷酸以其促进胃肠道发育与修复[1,2]、维持肝脏正常功能[3,4]、增强免疫能力[5]等方面的优势已成为医学研究的热点，目前国内外市场上出现了许多核酸保健食品以及核苷酸药品。但是对于外源摄入核苷酸是否会造成血清尿酸升高，进而导致高尿酸血症、痛风等疾病，目前还存在争议。本研究通过大鼠三代繁殖试验，观察外源性核苷酸对于啮齿类大鼠血清尿酸水平及肾脏的影响，从而分析判断摄食外源核苷酸是否会导致近期或远期的高尿酸血症及肾脏的损害，进而为核苷酸的安全性评价及核苷酸添加产品的开发提供科学依据。 材料与方法 　　一、材料 　　1、外源核苷酸：4种核苷酸比例为5′AMP∶5′CMP∶5′GMPNa2∶5′UMPNa2 =22.8∶25.8∶30.2∶20.