天荔汤对糖尿病大鼠肾脏氧化应激的影响_临床医学论文.docVIP

天荔汤对糖尿病大鼠肾脏氧化应激的影响_临床医学论文 天荔汤对糖尿病大鼠肾脏氧化应激的影响_临床医学论文 作者：陈国昌,张灼,彭海东,欧阳忠兴 【摘要】 目的 研究糖尿病大鼠肾脏氧化应激反应,观察天荔汤对氧化应激的影响。方法 将大鼠随机分为5组,即天荔汤高、低剂量组,西药对照组(Capotril+HB419),病理组和正常对照组。用四氧嘧啶(Alloxan)诱导糖尿病大鼠模型,8周后检测大鼠血糖、肾脏肥大、肾组织匀浆超氧化物歧化酶(SOD)、丙二醛(MDA)、谷胱甘肽过氧化酶(GSH-Px)的活性。结果 与正常组相比,病理组血糖、血脂、尿蛋白、肾重、体重等指标明显增高,肾匀浆MDA含量亦增加,而肾脏抗氧化酶的活性则降低,天荔汤显著降低肾组织匀浆SOD、MDA含量。结论 糖尿病大鼠肾脏存在氧化应激反应,肾组织抗氧化酶缺乏。经天荔汤治疗后上述变化明显被逆转。 【关键词】 糖尿病肾病；氧化应激；大鼠 　　Abstract：Objective To investigate oxidative stress in the kidney of diabetic rat in order to tobserve the effect of Tianli Decoction (TLT) on diabetic nephropathy. Methods The rats were devided into five groups：TLD-High dose group, TLD-Low dose group, Captopril (Cap) + HB419 group, pathologic control group, normal control group. Alloxan was used to induce the diabetic model of rats. After 8 weeks, blood glucose, blood lipid, urinary protein and the ratio of kidney weight to body weight in the rats of five groups were measured. The content of MDA and the renalintrinsic anti-oxidatantenzyme activities such as SOD and GSH-Px were evaluated. Results Comparing with normal group, the level of blood glucose, blood lipid, urinary protein and the ratio of kidney weight to body weight in diabetic group were significantly increased. The content of MDA in renalortex was increased, whereas the activities of anti-oxidatantenzyme were decreased. Conclusion Oxidativestress existed in the kidney of diabeticrats. The deficiency of intrinsic anti- oxidatantenzyme may contribute to the development of diabetic nephropathy. TLT can revers the obove changes. 　　Key words：diabetic nephropathy；oxidative stress；rat 我国北京地区的流行病学调查表明,糖尿病肾病(DN)在1型和2型糖尿病中分别占30%～40%与15%～20%[1]。本研究观察天荔汤对氧化应激反应的影响,以探讨其防治糖尿病肾病的作用机理。 　　1 材料与方法 　　1.1 动物 健康雄性Sprague-Dawley大鼠60只,体重在250～330 g之间,购自湖北省医药工业研究院,合格证号：医动字第14-001号。 　　1.2 药物 天荔汤：由天花粉、荔枝核、黄芪、知母、赤芍、葛根、丹参、女贞子、怀牛膝、生麦芽等多味中药组成,由湖北中医学院附属医院药房统一煎制成天荔合剂；优降糖,天津力生制药厂生产,批号9802010；卡托普利,常州制药厂生产,批号980302。 　　1.3 试剂与仪器 四氧嘧啶(Alloxan),Sigma公司产品,用0.9%氯化钠溶液低温(0～4℃)下溶解Alloxan,配成2%的