奶牛OLR1 3′UTR A223C多态的生物学信息分析.pdfVIP

ofAnhui 安徽农业科学，Journal A印．Sei．2011，3911)：75—77 责任编辑陈秀晨责任校对况玲玲 3’UTR 奶牛OLRl A223C多态的生物学信息分析 张传生1，耿立英1，尹春光2，曹顶国3，刘铮铸1，付志新1 (1．河北科技师范学院，河北昌黎066600；2．济宁学院。山东济宁273155；3．山东省农业科学院家禽研究所，山东济南250023) 3’uTR 摘要[目的]探索氧化型低密度脂蛋白受体OLRI A223C多态与奶牛乳脂率高低紧密关联的分子机制。[方法]用miRanda 3’L1’R (1．0)软件装载牛microRNA数据库，预测OLRl3’uTR潜在的micmRNA。[结果J共预测16个microRNA靶标，OLRlA223C突变 3’uTR 3’u’mA223C 定位于but．miR．370靶标种子序列的互补区。[结论】由于OLRlA—C突变导致bta-miR-370靶标的消失，为OLRl 多态与奶牛乳脂率高低紧密关联的分子机制深入研究提供铱据。 关键词OLRI；3’UTR；m／r一370；生物信息：microRNA 中图分类号S823．9“文献标识码A 文章编号0517—661l(2011)01—01X)75—03 BioinformaficsofA223C atthe3’UrR0f以冠1GeneinCow Analysis Polymorphism ZHANG etal Normal of O (Hebei 6l酿册) Chuan．sheng UniversityScieneeTechnology．Changli。Hebei Abstract T}leaimwasto the molecularmechanismsoftheA223C at3’UTRofoxidizedlOW lip小 Objective explore polymorphism density was relatedtomilkfat inCOW．IMethodThe microRNAlocatedinthe3’ proteinreceptor(OLRl)whichclosely percentage putative targets of UTRO脯1were witht}lemfi could themieroRNAdateaba．,pof Total16mieroRNAtar- predieted miRanda(1．0)whichcarry COW．『Result1 werefound．TheA223CSNPwaglocatedin ofbta—miR-370 3’UTRA_Cmu． gets complementaryregion targetsequence．『Conclusion]D脯l rationhadlcadtothe ofbta—miR一370 had basisforthefurther the disappearing target．whichprovide researchon molecuhrmechanismsofthe A223C milk in at3’UTRofOLRl whichwas relatedto fatpercentageCOW． polymorphism