烟草拮抗内生细菌的筛选与防病促生长效果.pdfVIP

48 Chinese Tobacco Science 2014-1235 6 1 2,3 4 1,2* 1. 650201 2. 650201 3. 650217 4. 651500 478 65 13.60%YN201408YN201442YN201448 YN201458 7 63.85%60.82%64.72%76.07%1.0×10 cfu/mL 30 min1 68.24%65.47%69.12 41.40%YN201448 5~6 10.95%29.31%67.24%16S rDNA YN201408YN201442 YN201448 YN201458 YN201448 S572.08 1007-5119201406-0048-06 DOI 10.13496/j.issn.1007-5119.2014.06.010 Screening, Growth Promotion, and Disease Control of Antagonistic and Endophytic Bacteria in Tobacco 1 2,3 4 1,2* YANG Zhenfu , WU Yixin , CHEN Yinglan , HE Yueqiu （1. National Engineering Center for Applied Techniques of Agricultural Biodiversity, Yunnan Agricultural University(YAU), Kunming 650201, China; 2. College of Agronomy and Biotechnology, YAU, Kunming 650201, China; 3. National and Local Joint Engineering Research Center for Screening and Application of Microbial Strains, Kunming 650217, China; 4. Extension Station of Agricultural Techniques, Luquan County, Luquan, Yunnan 651500, China ） Abstract: To obtain stable bacterial strains with good growth promotion of tobacco and black shrank disease control effect, 478 endophytic bacterial strains were isolated from tobacco leaves and stems. Among them 65 of strains ( accounting for 13.60%) could inhibit Phytophthora parasitica var. nicotianae by the traditional methodology. Especially, the four strains, YN201408, YN201442, YN201448 and YN201458 inhibited 63.85%, 60.82%, 64.72% and 76.07% of mycelia grow