复方连翘皮康乳膏中醋酸地塞米松的含量测定.pdfVIP

·1634 · Herald of Medicine Vol郾29 No郾12 December2010 复方连翘皮康乳膏中醋酸地塞米松的含量测定 赵维娟,王占庆,许摇 茜 (北京军区总医院药理科,100700) [摘摇 要]摇 目的摇 建立复方连翘皮康乳膏中醋酸地塞米松的含量测定方法。 方法摇 采用高效液相色谱(HPLC) 法,以ZORBAX SB鄄C 色谱柱(4.6 mm伊250 mm,5 滋m)为固定相,乙腈鄄水(52 48)为流动相,在240 nm 处测定样品含 18 量。 结果摇 醋酸地塞米松进样量在50~800 ng范围内呈良好的线性关系,平均回收率为101.32%,RSD=2.01%。 结论 摇 该方法测定制剂中醋酸地塞米松含量操作简便、快速,结果准确,适用于该制剂的质量控制。 [关键词]摇 地塞米松,醋酸;连翘皮康乳膏,复方;色谱法,高效液相;含量测定 [中图分类号]摇 R986;R927.2摇 摇 摇 [文献标识码]摇 A摇 摇 摇 [文章编号]摇 1004-0781(2010)12-1634-03 Determination ofDexamethasoneAcetonideinTransermalReleaseSolutionof Compound Lianqiaopikang Ointment ZHAO Wei鄄juan, WANG Zhan鄄qing,XU Qian(Department of Pharmacology, General Hospital of Beijing Military Command,Beijing 100700,China) ABSTRACT摇 Objective摇 To establishaHPLCmethodtodeterminedexamethasoneacetonideincompoundlianqiaopikang ointment. 摇 Methods摇 Contentswere determined by using a ZORBAX SB鄄C column (4.6 mm伊250 mm,5 滋m) as the fixed 18 phase,a mixtureof acetonitrileandpurifiedwater (52 48)asamobilephase,anddetectingat240 nmwavelength. 摇 Results 摇 The linear range of dexamethasone acetonidewas50-800 ng. The average recovery was 101.32% and RSD was2.01%. 摇 Conclusion摇 This method of determination of dexamethasone acetonide in compound lianqiaopikang ointment is rapid,simple and accurate,which fitting for quality control. KEY WORDS摇 Dexamethasone,acetonide;Lianqiaopikang ointment,compound;HPLC;Content determination 摇 摇 复方连翘皮康乳膏是由连翘、白鲜皮、荆芥穗等7 2摇 方法与结果摇 味中药提取物和醋酸地塞米松以适量辅料配伍而成的 2.1摇 色谱条件摇 色谱柱:ZORBAX SB鄄C (4.6 mm伊 18