特异性siRNA抑制牛MC4R基因在牛成纤维细胞表达效果地研究.pdfVIP

siRNA MC4R 1* 1 100193 MC4R 1808bp332 CDS 999bp5’596bp 3’214bp UTR MC4R CDS UTR siRNA MC4R MC4R InvitrogensiRNAMC4R NM_174110.1 4siRNApFU-GW-shMC4R Real-time PCR Western-blot4siRNA MC4R pFU-GW-shMC4R-1 -2-3-472 80%1RNA SYBR GreenPCR MC4RmRNAKD1NC P0.01 KD2 KD3 KD4NCP0.05 2 Western-Blot KD13 1.2 KD1NC(MC4R/GAPDH) e 1 v s i t C a 0.261.15(P 0.01) pFU-GW-shMC4R-1 l B 0.8 e F r A n s o 0.6 l KD1 MC4R N n l e R o c i 0.4 m s s R e 4 r p 0.2 C x M e 0 CON NC KD1 KD2 KD3 KD4 siRNA MC4R