放射性脑损伤和中药978-1防治机理系列研究.pdfVIP

放射性脑损伤及中药978-1 防治机理系列研究1[1] 1 2 1 2 朱广迎 蔡伟明 王德广 梁克 (1.徐州医学院肿瘤研究所，江苏徐州 221002 ；2. 中国协和医科大学肿瘤医院,北京100021) 摘要 目的 阐明照射后鼠学习记忆力损伤变化规律、大脑室管膜下区细胞放射损伤的剂量效应、相关基因表达及中药978-1 防 治机理。方法 麻醉后,采用直线加速器照射昆明鼠全脑,跳台法测定学习记忆力，并观测大脑室管膜下区细胞数、凋亡指数和p53 、 c-jun、c-fos 基因表达及中药978-1 对上述指标的影响。结果 ①照射后第2 、3、56 天鼠学习记忆力损伤明显。②照射后6 小时 室管膜下区细胞数开始下降,24 小时或48 小时达最低值,120 小时有所恢复;照射后2 小时室管膜下区细胞c-fos 表达率达高峰,6 小时明显下降,24 小时恢复到对照水平; 照射后2 小时c-jun 和p53 表达率均开始上升,分别于照射后6 小时、24 小时达高峰,48 小时下降,120 小时恢复。③照射后不同时间照射+ 中药组鼠学习记忆力损伤、室管膜下区细胞损伤均轻于单纯照射组。结论：① 照射后鼠学习记忆力损伤、室管膜下区细胞数和p53 、c-jun 基因过表达均有特定规律；②中药978-1 能防治放射性学习记忆力 损伤。 关键词 脑 辐射损伤 基因表达 中草药 中图分类号: 文献标识码:A 文章编号:1000-2065(2001) Experiment On Radiation Injury Of The Brain and its Prevention and treatment with traditional Chinese medicine of destagnation and bushen ZHU Guangying CAI Weiming Wang Deguang et al （Cancer Institute,Xuzhou Medical College,Xuzhou,Jiangsu 221002,China ） EXPERIMENT Objective To clarify the regularity of the injury of the Kunming mice learning and memory ability, correlative gene expression of subventricular cell(SVC) after irradiation and the usefulness of traditional Chinese medicine of destagnation and busheng to prevent or treat the radiation injury of the brain and correlative gene expression. Method After anaesthetized, the whole brain of Kunming mice was irradiated. Their learning and memory ability were measured with step down method. The cellularity, apoptotic index and expression rate of p53, c-jun and c-fos of SVC of the mice after irradiation were counted. The indexes of the mice with and without TCMDB after irradiation were compared. Results ①The second,third and fifty-sixth day after irradiation the learning and memory ability of mice was injured more appar