基于再生不定芽的蝴蝶兰继代扩繁体系建立.pdfVIP

中圊雇掌c亟搪 2015，3l(4)：162—166 ChineseAgriculturalScienceBulletin 基于再生不定芽的蝴蝶兰继代扩繁体系建立 彭 娇，崔金腾，王爱香，赵和文 (北京农学院园林学院，北京 102206) 摘 要：为降低蝴蝶兰通过再生原球茎途径频繁继代培养可能出现的变异及玻璃化风险、减轻继代培养 中的褐化 ，以蝴蝶兰花梗茎段再生的营养芽为试验材料 ，进行基于再生不定芽的继代扩繁及抗褐化研 究。结果显示，以5节花梗的中间腋芽茎段为材料，在vw+6-BA5mg／L+NAA0．1mg／L+蔗糖30g／L培 养基上进行启动培养，能获得最高的营养芽诱导率。继代培养中，TDZ促进再生不定芽的效果比6一BA 强。茎芽经过 “切叶”处理，在培养基vw+TDZ1mg／L+300mg／L柠檬酸+10g／L蔗糖+15％椰汁，或培 养基Vw+6-BA5mg／L+NAA0．1rag／L+300rng／L柠檬酸+10g／L蔗糖+15％椰汁上进行继代培养，能获 得最佳的继代扩繁效果。笔者研究认为初步建立了基于再生不定芽的蝴蝶兰继代扩繁体系。 关键词：蝴蝶兰；再生不定芽；继代扩繁 中图分类号：$682．31 文献标志码 ：A 论文编号：casbl4110138 BuildingofthePhalaenopsis’SubcultureMultiplicationSystem viaRegeneratingAdventitiousBuds PengJiao，CuiJinteng，WangAixiang，ZhaoHewen (CollegeofLandscape，BeijingUniversityofAgricuhure，Beijing102206) Abstract：Toreducethepossibilitiesofgeneticvariation orvitrification duringcontinuoussubculturevia regeneratingprotocormsandtoalleviatethebrowning，aPhalaenopsis’subculturetechniqueviaregenerating adventitiousbudsfrom vegetativebudsproducedrfom theflowerstalksandapplyinganti—browningagentwas studied．Theresultsshowed that：thehighestvegetativebud induction ratewasachievedwhen themiddle auxiliarybudstem segmentsfrom thefive—nodesflowerstalkswereinoculatedinthemedium ofVW +6一BA 5mg／L+NAA 0．1mg／L+ sugar30 g／L．Insubculture．TDZwasmoreeffectivethan 6一BA instimulating adventitious buds regeneration．The bestsubculture multiplication efficiency was achieved when the inoculatingbudstreatedwith c‘uttingleaf’werecultured inthemedium VW +TDZ 1mg／L+ citricacid 300rag／L+sugar10g／L+coconutwater15％．orinthemedium VW +6一BA 5mg／L+NAA 0．1mg／L+citric acid300mgCL+sugar10g／L+coconutwater15％．Through studies．thePhalaenopsis’subculture multiplicationsystem waspreliminarilysetupviaregeneratingadventitiousb