沙棘叶中黄酮提取条件优化.pdfVIP

沙棘叶中黄酮的提取条件优化 张宏伟、郑冬梅* 东北农业大学食品学院，哈尔滨150030 摘要：本研究使用盐酸水解乙醇超声法提取沙棘叶中沙棘黄酮，为开发和利用沙棘叶中沙棘 黄酮资源提新的方法。研究采用盐酸水解乙醇超声法从沙棘叶中提取黄酮，使用高效液相色 谱法测定提取液中槲皮素和异鼠李素的含量。研究乙醇浓度、盐酸浓度、水解时间、加热时 间和料液等因素对沙棘叶中槲皮素和异鼠李素提取的影响。研究得到最优工艺参数为乙醇浓 度70%，水解时间35min，盐酸浓度25% ，水解温度60℃，料液比1:20。研究结果表明， 盐酸水解乙醇超声提取沙棘叶中黄酮可行，本方法有助于深入开发和利用沙棘资源。 关键词：沙棘叶；沙棘黄酮；酸水解；超声提取 Optimization of Extraction Process of Flavonoids form Sea Buckthorn Leaves Zhang Hongwei 、Zheng Dongmei* Food Department of NortheastAgricultural University, Harbin 150030 Abstract: In order to provide a new approach for development and utilization of the sea buckthorn flavonoids in sea buckthorn leaves, the method of hydrochloric acid and ethanol ultrasonic extraction of sea buckthorn flavonoids in sea buckthorn leaves pectin and its emulsion properties were investigated. Ultrasonic method using hydrochloric acid and ethanol extract from the leaves of sea buckthorn flavonoids, high performance liquid chromatography method was used in the content of quercetin and isorhamnetin. Of ethanol, hydrochloric acid concentration, hydrolysis time, heating time and solid to liquid and other factors on sea buckthorn leaf extract quercetin and isorhamnetin impact. The optimal process parameters of the ethanol concentration of 70%, hydrolysis time 35min, hydrochloric acid concentration of 25%, hydrolysis temperature is 60 ℃, solid to liquid ratio of 1:20. The results showed that hydrochloric acid ethanol extraction of sea buckthorn leaves flavonoids ultrasound is feasible, this approach helps to further the development and use of sea buckthorn resources. Key words: sea buckthorn leaf; sea buckthorn flavonoids; acid hydrolysis; ultrasonic extraction 0 前言 沙棘果和叶中黄酮类主要成分为槲皮素、异鼠李素、山奈酚及其苷类、杨梅酮、氯原酸、 猪草苷、儿茶酸、黄芪苷等。沙棘黄酮具有抗肿瘤，保护心血管系统，提高机体免疫，抗氧 [1-3] 化，镇痛等作用 。陈金娥等（2007 ）研究微波萃取法浸