威灵仙总皂苷抗类风湿性关节炎的作用机制_李特.pdfVIP

学 报 Journal of Ch ina PharmaceuticalUniversity 2009, 40( 2) : 157 - 1 0 157 * 李 特, 李运曼 , 刘丽芳, 王宇新, 李丰文, 朱兴祥 (, 210009) : 对威灵仙总皂苷(TSRC)抗类风湿关节炎的作用机制进行研究: 利用刀豆蛋白 A ( ConA) 诱导小 T 淋巴细胞增殖模型; 利用大 IL-1BIL-2TNF-A 定量酶联检测试剂盒测定原发性佐剂性关节炎大 血清中 IL-1BIL- 2TNF-A 的含量; 利用氢氧化钾-甲醇溶液法测定原发性佐剂性关节炎大 肿胀足前列腺素 E ( PEG )的含量: TSRC 2 2 能抑制 ConA诱导小 T 淋巴细胞的增殖; 降低大 佐剂性关节炎细胞因子 ( IL-1BIL-2TNF-A)的含量; 降低佐剂性关节 炎大 炎性肿胀足 PGE 的含量: TSRC 可能是通过抑制T 淋巴细胞的过度增殖抑制细胞因子和 PEG 来发挥抗类 2 2 风湿性关节炎作用 威灵仙总皂苷; 类风湿性关节炎; T 淋巴细胞; 白介素; 肿瘤坏死因子-A; 前列腺素 E2 R9 5 A 1000 - 5048( 2009) 02 - 0157- 04 M echanism of total saponins of Radix Clematidis on rheumatoid arthritis * L ITe, LIYun-man , L IU L-i fang, WANG X in-yu, LI Feng-w en, ZHU X ing-xiang D epartm ent of Physiology, Ch ina Pharmaceu tical University, N anj ing 210009, Ch ina Abstract A im:To study the action mechanism of total saponinis ofRadix Clematidis (TSRC) on rheumatoid ar- thritis (RA ). M ethods: The proliferation of ConA-induced T lymphocytemodelw as observed to find outw hether TSRC had som e effect on the proliferation ofT lymphocytes; The serum concentrations of interleuk in-1 ( IL-1B ), interleuk in-2 ( IL-2) andTNF-A in adjuvant-induced prmi ary arthritis rats w erem easured by the enzyme-linked mi munoadsorbent assay (ELISA ) kits1And the concentrations of PGE in the sw ollen feet of adjuvant-induced 2 prmi ary arthritis ratsw ere measured by the m ethod of potassium hydrox ide-methanol1Results: It w as found that TSRC inhib ited the p