TNF-α基因多态性与类风湿性关节炎相关性研究.pdfVIP

2015往 临床血液学杂志 28卷 2期 JClinHematol(China) ·97 · TNF—基因多态性与类风湿性关节炎相关性研究 王平 贺艳丽 曾筱倩 王利民 高云 胡丽华 [摘要] 目的：探讨肿瘤坏死 因子一n(TNF-~)启动子区一308基因多态性与湖北汉族人类风湿性关节炎 (RA) 易感性的关系。方法：用聚合酶链反应和限制性片段长度多态性 (PCR／RFLP)的方法对 113例 RA 患者及 126 例健康人进行 TNF—a的-308位点多态性分析。结果：RA组与对照组 TNF．a的一308位点的基 因型频率和等位基 因频率 G／G、G／A、A／A之间差异无统计学意义(P0．05)；并且 TNF_a的一308位点的基 因多态性与 RA活动期 指标ESR和CRP高低无关。结论：TNF—a启动子区一308基因多态性不是类风湿性关节炎易感性和严重程度的 一 个风险 因子 。 [关键词] 肿瘤坏死 因子 ；类风湿性关节炎；基 因多态性 doi：10．13201／J．issn．1004—2806一b．2015．02．003 [中图分类号] R593．22 [文献标志码] A Correlationbetweentumornecrosisfactor一仅genepolymorphism andsusceptibilitVofrheumatoidarthritis W_ANG Ping HE Yanli ZENG Xiaoqian W_ANG Lim in GA0 Yun HU Lihua (DepartmentofClinicalLaboratory，UnionHospital，TongJiMedicalCollege，HuazhongUni— versityofScienceandTechnology，W uhan，430022，China；CenterforStem CellResearch and Application，UnionHospital，TongjiMedicalCollege，HuazhongUniversityofScienceand Technology) Abstract Objective：Toinvestigatethecorrelationbetweentumornecrosisfactor—alpha(TNF—a)一308(GA) genepolymorphism andsusceptibilityofrheumatoidarthritis(RA)．M ethod：Thepolymorphism intheTNF-a一308 weredeterminedbyPCR—RFLPin113Rheumatoidarthritispatientsand126controls．Result：Therewasnosignif— icantdifferenceintheallelefrequencyof—。808(G／A)siteofTNF—。agenepromoterregionandgenotypefrequencyG／ G，G／A，A／A betweenRA patientsandcontrols(P0．05)．Moreover，therewasnoassociationbetweenTNF—a genotypesanddiseaseactivitymeasures，includingtheleveloferythrocytesedimentationrate (ESR)andC-reac— tiveprotein (CRP)．Conclusion：TNF-a一308 promoterpolymorphism wouldbenotageneticriskfactorforRA susceptibilityandseverity． Keywords tumornecrosisfactor；Rheumatoidarth