血吸虫种株随机扩增多态性DNA差异研究.pdfVIP

2001, 17 (4) Chi ese Jour al of Zoo oses 49 : 1002- 2694( 2001) 04- 0049- 05 血吸虫种株随机扩增多态性DNA 差异研究 冯友仁 牛安欧 方正明 : 对曼氏血吸虫日本血吸虫的种株基因组 DNA 进行多态性分析 随机扩增多态性DNA 技术 ( RAPD) 日本血吸虫与曼氏血吸虫种间差异 显, 两种间的遗传距离大于0. 90; 日本血吸虫中国大陆株与台湾株之 间存在较大的差异, 两者的遗传距离大于0. 20; 日本血吸虫中国大陆株内, 以四川天全为代表的西部山区型地域株与湘鄂 赣代表的湖沼型地域株之间有一定差异, 其遗传距离在0. 060~ 0.067 之间; 湘鄂赣湖区之间并非完全一样, 也存在较小的 差异, 遗传距离在0.000~ 0. 029 之间; 位于湖北省境内的4 个地域株的日本血吸虫存在一定的基因差异; RAPD 技术能鉴别 血吸虫雌雄性别差异, 718bp 为雌虫特异扩增条带 日本血吸虫中国大陆株已发生不同程度的遗传分化, 中国大陆株 并非单一的地域株, 是由若干不同株构成的复合株; 湖北省血吸虫虫株至少存在3 个基因类型; RAPD 技术能从 DNA 基因水 平区分血吸虫的种株及性别差异, 是分析血吸虫基因遗传变异的好方法 : 日本血吸虫; 曼氏血吸虫; RAPD; 遗传变异; 遗传分化; 种株 STUDIES ON THE DIFFERENCES OF SCHISTOSOMA SPECIES AND STRAINS BY USING RANDOM AMPLIFIED POLYMORPHICDNA MARKERS FENG Youre , NIU A ou, FANG Zhe gmi g ( Dep ar tment of P ar asitology , Tongj i M ed ical Univ ersity Wuhan 430030) ABSTRACT:Aim I order to exami e DNA polymorphisms betwee species, strai s a d sexes of S . manson i a d S . j ap on ic um . Methods To use a ra dom amplified polymorphic DNA tech ique( RAPD) . Results Highly variable producte partter s were observed betw ee S . manson i a d S . j ap on icum by 6 primers. Some of t he tested primers, B5, A8, produced partter s i cluded ba ds t hat were polymorphic betwee strai s of mai la d a d T aiwa i Chi a. Mi or differe ces were observed betwee male a d female adult, fragme t of 718 bp was reported as sex marker . Data were a alysed by ge et ic dista ce( D) , a d ge et ic similarit y( S) . Ge etic dista ce( D) betwee tw o species was more tha 0. 90; ge etic dista ce( D) betwee mai la d a d Taiw a strai s of S . j ap on icum w as more tha 0. 20; 6 strai s from marshla d- lake regio s, they were ra ged from 0.000 to 0. 029, betwee marshla d - lake re gio s a d mou tai ous- hilly regio s ra